Myc Tag (AA 410-419) Antikörper (Biotin)

Details zu Produkt Nr. ABIN1103150, Anbieter: Anmelden zum Anzeigen
Antigen
  • MRTL
  • MYCC
  • bHLHe39
  • c-Myc
  • ARC
  • B430311C09Rik
  • MYC
  • NOP
  • Nop30
  • MYC proto-oncogene, bHLH transcription factor
  • nucleolar protein 3 (apoptosis repressor with CARD domain)
  • MYC
  • Nol3
Epitop
AA 410-419
55
20
18
10
6
4
2
2
2
2
1
1
Reaktivität
Tag
211
58
14
12
6
5
3
1
1
Wirt
Kaninchen
167
63
20
17
6
5
3
1
Klonalität
Polyklonal
Konjugat
Biotin
28
23
15
9
5
5
4
4
4
4
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
239
117
95
69
49
39
36
36
23
7
6
5
5
4
4
4
3
3
3
2
1
1
1
1
1
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen This antibody was purified from whole rabbit serum prepared by repeated immunizations with Myc epitope tag peptide E-Q-K-L-I-S-E-E-D-L conjugated to KLH using maleimide. The sequence corresponds to aa 410-419 of human c-Myc.

Type of Immunogen: Purified protein
Isotyp IgG
Spezifität This affinity purified antibody is directed against human c-myc and is useful in determining its presence in various assays. This polyclonal anti-Myc-tag antibody detects overexpressed proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-Rabbit Serum.
Reinigung Affinity purified
Substanzklasse Tag
Applikationshinweise Approved: ELISA, IHC (1:1000 - 1:5000), WB (1:2000 - 1:10000)

Usage: Anti-myc has utility to detect the fusion protein of the myc epitope cloned along with the target gene. As such, anti-myc/myc can be used to identify fusion proteins containing the myc epitope. The antibody recognizes the Myc tag fused either to the amino- or carboxy- termini of targeted proteins. This antibody has been tested by ELISA and western blotting against both the immunizing peptide and myc containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry. Biotin conjugated anti-EPITOPE tag antibodies are generally suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Rekonstitution deionized water
Konzentration Lot specific
Buffer 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 1 % BSA, 0.01 % sodium azide
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung avoid freeze thaw cycles.
Lagerung 4 °C,-20 °C
Informationen zur Lagerung Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
Bilder des Herstellers
 image for anti-Myc Tag (AA 410-419) antibody (Biotin) (ABIN1103150) anti-Myc Tag (AA 410-419) antibody (Biotin)
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