Interferon gamma Antikörper

Produktnummer ABIN1102596

Produktdetails anti-Interferon gamma Antikörper

Target: Interferon gamma (IFNG)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Interferon gamma Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Neutralization (Neut), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human IFN-gamma

Aufreinigung: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human IFN-gamma.

Type of Immunogen: Cell extract

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (0.5 - 2 μg/mL), IHC, IHC-P (5 μg/mL), Neut, WB

Usage: Immunohistochemistry: LS-B7487 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 μg/mL is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human IFN-gamma (10 ng/mL), a concentration of 0.13-0.20 μg/mL of this antibody is required. ELISA: To detect Human IFN-gamma by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IFN-gamma (LS-C104708) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human IFN-gamma. Western Blot: To detect Human IFN-gamma by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IFN-gamma is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Sterile water

Konzentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handhabung: Avoid freeze/thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Details zu Interferon gamma

Target: Interferon gamma (IFNG)

Andere Bezeichnung: IFN Gamma / Interferon Gamma (IFNG Produkte)

Synonyme: IFG antikoerper, IFI antikoerper, IFN-g antikoerper, Ifg antikoerper, IFNG2 antikoerper, IFN-gamma antikoerper, IFN-G antikoerper, IFNG antikoerper, IFNgamma antikoerper, TCRalpha antikoerper, INF-G antikoerper, ifng antikoerper, interferon gamma antikoerper, interferon, gamma 1-2 antikoerper, IFNG antikoerper, Ifng antikoerper, ifng1-2 antikoerper

Hintergrund: Name/Gene ID: IFNG
Family: Interferon

Synonyms: IFNG, IFG, Immune interferon, IFI, Interferon gamma, IFN-gamma, Interferon, gamma

Gen-ID: 3458

UniProt: P01579

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagie