IL-4 Antikörper

Produktnummer ABIN1102500

Produktdetails anti-IL-4 Antikörper

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser IL-4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Neutralization (Neut), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human IL-4

Aufreinigung: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human IL-4.

Type of Immunogen: Cell extract

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (0.5 - 2 μg/mL), IHC, IHC-P (5 μg/mL), Neut (3 - 5 μg/mL), SwELISA, WB

Usage: Immunohistochemistry: LS-B7474 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 μg/mL is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hIL-4 (1.50 ng/mL), a concentration of 03-05 μg/mL of this antibody is required. ELISA: To detect hIL-4 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-4 (LS-C104301) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-4. Western Blot: To detect hIL-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-4 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Sterile water

Konzentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handhabung: Avoid freeze/thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Details zu IL-4

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Andere Bezeichnung: IL4 (IL4 Produkte)

Synonyme: IL4 antikoerper, IL-4 antikoerper, BCGF-1 antikoerper, BCGF1 antikoerper, BSF-1 antikoerper, BSF1 antikoerper, Il-4 antikoerper, Il4e12 antikoerper, IL2 antikoerper, interleukin 4 antikoerper, IL4 antikoerper, Il4 antikoerper

Hintergrund: Name/Gene ID: IL4
Family: Hormone

Synonyms: IL4, B cell growth factor 1, B-cell stimulatory factor 1, B_cell stimulatory factor 1, BCGF1, BSF-1, Binetrakin, BCGF-1, Interleukin 4, Interleukin-4, Pitrakinra, BSF1, IL-4

Gen-ID: 3565

UniProt: P05112

Pathways: JAK-STAT Signalweg, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation