His Tag Antikörper

Details zu Produkt Nr. ABIN1102044, Anbieter: Anmelden zum Anzeigen
Antigen
Reaktivität
Tag
624
28
15
5
4
1
Wirt
Maus
413
189
27
23
8
8
3
2
Klonalität (Klon)
Monoklonal ()
Konjugat
Unkonjugiert
58
46
37
17
13
13
10
10
10
9
9
9
8
8
8
7
7
7
5
4
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Applikation
ELISA, Western Blotting (WB)
569
405
165
109
83
82
79
27
13
11
8
7
6
6
5
5
4
4
4
4
2
2
1
1
1
1
1
1
1
1
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen HIS Tag antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.

Type of Immunogen: Hapten
Sequenz HHHHHH
Klon 33D10-D2-G8
Isotyp IgG1
Spezifität 6X HIS Epitope Tag antibody is directed against the 6X His motif and is useful in determining its presence in various assays. This monoclonal anti-6X His tag antibody detects over-expressed proteins containing the 6X His epitope tag. To date, this antibody has reacted with all His tagged proteins so far tested. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins. The antibody recognizes the His-tag (His-His-His-His-His-His) fused to either the amino- or carboxy-termini of targeted proteins in transfected or transformed cells.
Reinigung Purified
Sterilität Sterile filtered
Substanzklasse Tag
Applikationshinweise Approved: ELISA, WB (1:1000)

Usage: Anti-6X His is optimally suited for monitoring expression of His-tagged fusion proteins. As such, anti-6X His/6X His can be used to identify fusion proteins that contain the 6X His epitope. The antibody recognizes the His tag fused either to the amino- or carboxy- termini of targeted proteins. This antibody has been tested by ELISA and western blotting against both the immunizing peptide and His-containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration Lot specific
Buffer 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01 % sodium azide, sterile filtered
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung avoid freeze thaw cycles.
Lagerung 4 °C,-20 °C
Informationen zur Lagerung Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
Bilder des Herstellers
 image for anti-His Tag antibody (ABIN1102044) anti-His Tag antibody
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