SLC1A3 Antikörper (Cytoplasmic Domain)
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- Target Alle SLC1A3 Antikörper anzeigen
- SLC1A3 (Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3))
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Bindungsspezifität
- Cytoplasmic Domain
- Reaktivität
- Human, Ratte, Maus, Affe, Hamster, Rind (Kuh), Pferd, Schwein, Kaninchen
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser SLC1A3 Antikörper ist unkonjugiert
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Applikation
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Marke
- IHC-plus™
- Spezifität
- Human SLC1A3 / GLAST. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- Homologie
- Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Bovine, Horse, Rabbit, Pig (100%) Panda, Dog, Opossum, Guinea pig, Turkey, Chicken (95%) Xenopus (84%).
- Aufreinigung
- Immunoaffinity purified
- Immunogen
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Synthetic 19 amino acid peptide from cytoplasmic domain of human SLC1A3 / GLAST. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Bovine, Horse, Rabbit, Pig (100%), Panda, Dog, Opossum, Guinea pig, Turkey, Chicken (95%), Xenopus (84%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product SLC1A3 Primärantikörper
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- Applikationshinweise
- Approved: IHC, IHC-P (5 μg/mL)
- Kommentare
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Target Species of Antibody: Human
- Testdurchführung
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- Lot specific
- Buffer
- PBS, less than 0.1 % sodium azide.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- Haltbarkeit
- 12 months
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- Target
- SLC1A3 (Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3))
- Andere Bezeichnung
- SLC1A3 / GluT-1 / GLAST (SLC1A3 Produkte)
- Synonyme
- AF001784 antikoerper, CG3747 antikoerper, Dglt-1 antikoerper, Dglut-1 antikoerper, Dmel\\CG3747 antikoerper, EAAT antikoerper, EAAT1 antikoerper, dEAAT antikoerper, dEAAT1 antikoerper, dEaat1 antikoerper, slc1a3 antikoerper, wu:fb98e05 antikoerper, wu:fd50b05 antikoerper, zgc:56661 antikoerper, zgc:77768 antikoerper, SLC1A3 antikoerper, GLAST antikoerper, GluT-1 antikoerper, EA6 antikoerper, GLAST1 antikoerper, AI504299 antikoerper, B430115D02Rik antikoerper, Eaat1 antikoerper, GLAST-1 antikoerper, GLU-T antikoerper, Gmt1 antikoerper, MGluT1 antikoerper, Excitatory amino acid transporter 1 antikoerper, solute carrier family 1 member 3 antikoerper, excitatory amino acid transporter 1 antikoerper, solute carrier family 1 (glial high affinity glutamate transporter), member 3a antikoerper, solute carrier family 1 (glial high affinity glutamate transporter), member 3 antikoerper, Eaat1 antikoerper, SLC1A3 antikoerper, PTRG_00379 antikoerper, Tsp_02392 antikoerper, slc1a3a antikoerper, Slc1a3 antikoerper
- Hintergrund
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Name/Gene ID: SLC1A3
Subfamily: Sodium:dicarboxylate symporter
Family: Transporter
Synonyms: SLC1A3, EA6, EAAT-1, EAAT1, GLAST, GLAST1, GLAST-1, GLUT-1 - Gen-ID
- 6507
- Pathways
- Sensory Perception of Sound, Synaptic Membrane, Dicarboxylic Acid Transport
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