SLC1A3 Antikörper (C-Term)

Produktnummer ABIN3044001

Produktdetails anti-SLC1A3 Antikörper

Target: SLC1A3 (Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3))

Bindungsspezifität: AA 519-537, C-Term

Reaktivität: Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SLC1A3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Verwendungszweck: Rabbit IgG polyclonal antibody for Excitatory amino acid transporter 1(SLC1A3) detection. Tested with WB, IHC-P, IHC-F in Human,Mouse,Rat.

Sequenz: MKKPYQLIAQ DNETEKPID

Kreuzreaktivität (Details): Predicted Cross Reactivity: human
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Produktmerkmale: Rabbit IgG polyclonal antibody for Excitatory amino acid transporter 1(SLC1A3) detection. Tested with WB, IHC-P, IHC-F in Human,Mouse,Rat.
Gene Name: solute carrier family 1(glial high affinity glutamate transporter), member 3
Protein Name: Excitatory amino acid transporter 1

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human EAAT1(519-537aa MKKPYQLIAQDNETEKPID), different from the related rat and mouse sequences by three amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse, Rat, Predicted Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Human
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and IHC(F).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Referenzen für anti-SLC1A3 Antikörper (ABIN3044001)

Ho, Hartley, Flaherty, Rajarajan, Abdelaal, Obiorah, Barretto, Muhammad, Phatnani, Akbarian, Brennand: "Evaluating Synthetic Activation and Repression of Neuropsychiatric-Related Genes in hiPSC-Derived NPCs, Neurons, and Astrocytes." in: Stem cell reports, Vol. 9, Issue 2, pp. 615-628, (2018) (PubMed).

Ding, Zhang, Liu, Zhang, Ma, Bruce, Zhang: "Tumor necrosis factor-? promotes the expression of excitatory amino-acid transporter 2 in astrocytes: Optimal concentration and incubation time." in: Experimental and therapeutic medicine, Vol. 8, Issue 6, pp. 1909-1913, (2014) (PubMed).

Details zu SLC1A3

Target: SLC1A3 (Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3))

Andere Bezeichnung: SLC1A3 (SLC1A3 Produkte)

Synonyme: AF001784 antikoerper, CG3747 antikoerper, Dglt-1 antikoerper, Dglut-1 antikoerper, Dmel\\CG3747 antikoerper, EAAT antikoerper, EAAT1 antikoerper, dEAAT antikoerper, dEAAT1 antikoerper, dEaat1 antikoerper, slc1a3 antikoerper, wu:fb98e05 antikoerper, wu:fd50b05 antikoerper, zgc:56661 antikoerper, zgc:77768 antikoerper, SLC1A3 antikoerper, GLAST antikoerper, GluT-1 antikoerper, EA6 antikoerper, GLAST1 antikoerper, AI504299 antikoerper, B430115D02Rik antikoerper, Eaat1 antikoerper, GLAST-1 antikoerper, GLU-T antikoerper, Gmt1 antikoerper, MGluT1 antikoerper, Excitatory amino acid transporter 1 antikoerper, solute carrier family 1 member 3 antikoerper, excitatory amino acid transporter 1 antikoerper, solute carrier family 1 (glial high affinity glutamate transporter), member 3a antikoerper, solute carrier family 1 (glial high affinity glutamate transporter), member 3 antikoerper, Eaat1 antikoerper, SLC1A3 antikoerper, PTRG_00379 antikoerper, Tsp_02392 antikoerper, slc1a3a antikoerper, Slc1a3 antikoerper

Hintergrund: Solute carrier family 1(glial high-affinity glutamate transporter), member 3, also known as SLC1A3, EAAT1 or GLAST, is a protein that in humans is encoded by the SLC1A3 gene. This gene is a member of high affinity glutamate transporter family. SLC1A3 is mapped to chromosome 5p13.2 by fluorescence in situ hybridization(FISH). This gene transports L-glutamate and also L- and D-aspartate. It is essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. This gene acts as a symport by cotransporting sodium.

Synonyms: EA6 antibody|EAA1_HUMAN antibody|EAAT1 antibody|Excitatory amino acid transporter 1 antibody|FLJ25094 antibody|GLAST antibody|GLAST-1 antibody|GLAST1 antibody|Glial high affinity glutamate transporter antibody|glutamate/aspartate transporter, high affinity, sodium-dependent antibody|High affinity neuronal glutamate transporter antibody|Slc1a3 antibody|Sodium dependent glutamate/aspartate transporter antibody|Sodium-dependent glutamate/aspartate transporter 1 antibody|Solute carrier family 1(glial high affinity glutamate transporter) member 3 antibody|Solute carrier family 1 member 3 antibody

UniProt: P43003

Pathways: Sensory Perception of Sound, Synaptic Membrane, Dicarboxylic Acid Transport