P2RY8 Antikörper (C-Term)
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- Target Alle P2RY8 Antikörper anzeigen
- P2RY8 (Purinergic Receptor P2Y, G-Protein Coupled, 8 (P2RY8))
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Bindungsspezifität
- C-Term
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Reaktivität
- Human
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser P2RY8 Antikörper ist unkonjugiert
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Applikation
- Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Spezifität
- Human P2RY8 / P2Y8. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- Homologie
- Percent identity with other species by BLAST analysis: Human (100%) Gorilla (95%).
- Aufreinigung
- Immunoaffinity Chromatography
- Immunogen
- Synthetic 20 amino acid peptide from C-terminus of human P2RY8 / P2Y8.
- Top Product
- Discover our top product P2RY8 Primärantikörper
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- Kommentare
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This antibody has been further validated specifically for use under standardized IHC conditions against formalin-fixed paraffin-embedded human tissues.
- Testdurchführung
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Konzentration
- Lot specific
- Buffer
- PBS, 0.1% sodium azide.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C/-80 °C
- Informationen zur Lagerung
- Long term: -70°C, Short term: +4°C
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- Target
- P2RY8 (Purinergic Receptor P2Y, G-Protein Coupled, 8 (P2RY8))
- Andere Bezeichnung
- P2RY8 / P2Y8 (P2RY8 Produkte)
- Synonyme
- P2Y8 antikoerper, P2Y purinoceptor 8 antikoerper, P2Y receptor family member 8 antikoerper, purinergic receptor P2Y8 antikoerper, p2ry8 antikoerper, P2RY8 antikoerper
- Hintergrund
- Standard Gene Symbol: P2RY8, Gene Family: GPCR, Gene Subfamily: Orphan-A, Synonyms: P2RY8, p2RY8, p2Y purinoceptor 8, p2Y8
- Gen-ID
- 286530
- UniProt
- Q86VZ1
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