NR5A1 Antikörper (Internal Region)
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- Target Alle NR5A1 Antikörper anzeigen
- NR5A1 (Nuclear Receptor Subfamily 5, Group A, Member 1 (NR5A1))
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Bindungsspezifität
- Internal Region
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Reaktivität
- Human, Affe, Fledermaus
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser NR5A1 Antikörper ist unkonjugiert
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Applikation
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Marke
- IHC-plus™
- Spezifität
- Human NR5A1. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except TRIM23 (47 % ).
- Homologie
- Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Bat (100%) Elephant, Panda, Dog, Bovine, Rabbit, Horse, Pig (94%) Mouse, Rat, Hamster (88%) Turkey, Chicken, Platypus, Xenopus (82%).
- Aufreinigung
- Immunoaffinity purified
- Immunogen
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Synthetic 17 amino acid peptide from internal region of human NR5A1. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Bat (100%), Elephant, Panda, Dog, Bovine, Rabbit, Horse, Pig (94%), Mouse, Rat, Hamster (88%), Turkey, Chicken, Platypus, Xenopus (82%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product NR5A1 Primärantikörper
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- Applikationshinweise
- Approved: IHC, IHC-P (2 - 18 μg/mL)
- Kommentare
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Target Species of Antibody: Human
- Testdurchführung
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- Lot specific
- Buffer
- PBS, less than 0.1 % sodium azide.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- Haltbarkeit
- 12 months
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- Target
- NR5A1 (Nuclear Receptor Subfamily 5, Group A, Member 1 (NR5A1))
- Andere Bezeichnung
- NR5A1 / SF1 (NR5A1 Produkte)
- Synonyme
- AD4BP antikoerper, ELP antikoerper, FTZ1 antikoerper, FTZF1 antikoerper, POF7 antikoerper, SF-1 antikoerper, SF1 antikoerper, SPGF8 antikoerper, SRXY3 antikoerper, Ad4BP antikoerper, ELP-3 antikoerper, Ftz-F1 antikoerper, Ftzf1 antikoerper, Ad4BP/SF-1 antikoerper, Ad4bp antikoerper, Sf-1 antikoerper, NR5A1 antikoerper, sf-1 antikoerper, elp antikoerper, sf1 antikoerper, ftz1 antikoerper, ad4bp antikoerper, ftzf1 antikoerper, SF-1/Ad4BP antikoerper, SI:zC167P9.2 antikoerper, ff1d antikoerper, nr5a2l antikoerper, nuclear receptor subfamily 5 group A member 1 antikoerper, nuclear receptor subfamily 5, group A, member 1 antikoerper, nuclear receptor subfamily 5 group A member 1 L homeolog antikoerper, nuclear receptor subfamily 6 group A member 1 antikoerper, nuclear receptor subfamily 5, group A, member 1b antikoerper, NR5A1 antikoerper, Nr5a1 antikoerper, nr5a1.L antikoerper, nr5a1 antikoerper, NR6A1 antikoerper, nr5a1b antikoerper
- Hintergrund
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Name/Gene ID: NR5A1
Subfamily: NR5 Fushi tarazu-like
Family: NHR
Synonyms: NR5A1, AD4BP, Adrenal 4 binding protein, Adrenal 4-binding protein, ELP, Steroidogenic factor-1, SRXY3, Steroidogenic factor 1, Nuclear receptor AdBP4, Nuclear receptor elp, POF7, SF-1, SPGF8, Steroid hormone receptor Ad4BP, STF-1, FTZ1, FTZF1, Fushi tarazu factor homolog 1 - Gen-ID
- 2516
- Pathways
- Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Maintenance of Protein Location
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