HTR7 Antikörper (N-Term)

Produktnummer ABIN1048278

Produktdetails anti-HTR7 Antikörper

Target: HTR7 (5-Hydroxytryptamine (serotonin) Receptor 7 (Adenylate Cyclase-Coupled) (HTR7))

Bindungsspezifität: N-Term

Reaktivität: Human, Hund, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HTR7 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)

Marke: IHC-plus™

Spezifität: Human 5HT7 Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Homologie: Percent identity with other species by BLAST analysis: Human, Marmoset, Dog (100%) Mouse, Rat, Hamster, Panda (95%) Gorilla, Pig (90%) Horse (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic 20 amino acid peptide from N-terminal extracellular domain of human 5HT7 Receptor. Percent identity with other species by BLAST analysis: Human, Marmoset, Dog (100%), Mouse, Rat, Hamster, Panda (95%), Gorilla, Pig (90%), Horse (85%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (10 μg/mL)

Kommentare:

Target Species of Antibody: Human

Testdurchführung:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu HTR7

Target: HTR7 (5-Hydroxytryptamine (serotonin) Receptor 7 (Adenylate Cyclase-Coupled) (HTR7))

Andere Bezeichnung: HTR7 / 5-HT7 Receptor (HTR7 Produkte)

Synonyme: 5-HT7 antikoerper, 5-HT-dro antikoerper, 5-HT-dro1 antikoerper, 5-HT7Dro antikoerper, 5-HT[7]Dro antikoerper, 5-HT[[7Dro]] antikoerper, 5-HT[[7]] antikoerper, 5-HT[[7]]Dro antikoerper, 5-HT[[dro1]] antikoerper, 5-HTdro1 antikoerper, 5HT-7 antikoerper, 5HT-R1 antikoerper, 5HT-dro antikoerper, 5HT-dro1 antikoerper, 5HT[[7Dro]] antikoerper, 5htdror antikoerper, CG12073 antikoerper, DRO1 antikoerper, Dm5HTdro1 antikoerper, Dmel\\CG12073 antikoerper, 5Ht7 antikoerper, 5HT7 antikoerper, htr7-A antikoerper, 5-hydroxytryptamine receptor 7 antikoerper, 5-hydroxytryptamine (serotonin) receptor 7 antikoerper, 5-hydroxytryptamine (serotonin) receptor 7, adenylate cyclase-coupled antikoerper, 5-hydroxytryptamine (serotonin) receptor 7, adenylate cyclase-coupled L homeolog antikoerper, HTR7 antikoerper, 5-HT7 antikoerper, LOC752003 antikoerper, Htr7 antikoerper, htr7.L antikoerper

Hintergrund: Name/Gene ID: HTR7
Subfamily: Serotonin
Family: GPCR

Synonyms: HTR7, 5-HT7, 5-HT7 receptor, 5-HT-7, 5-hydroxytryptamine 7 receptor, 5HT7, 5-HT-X, 5-hydroxytryptamine receptor 7, 5HT7 Receptor, HT7, Serotonin 5-HT7 receptor, Serotonin receptor 7

Gen-ID: 3363

Pathways: JAK-STAT Signalweg