Kaninchen anti-Maus IgG (Chain gamma) Antikörper (Atto 550)

Details zu Produkt Nr. ABIN1043989, Anbieter: Anmelden zum Anzeigen
Antigen
Epitop
Chain gamma
6368
2017
1755
1137
784
649
374
302
35
34
31
15
11
7
3
3
1
1
1
1
1
1
1
Reaktivität
Maus
3054
2854
2344
1624
1463
773
740
499
484
423
403
401
311
295
274
159
151
111
49
34
30
26
16
15
11
10
8
7
5
5
4
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
Wirt
Kaninchen
7023
5235
1915
1077
508
358
89
84
52
35
23
15
8
5
3
1
1
Klonalität
Polyklonal
Konjugat
Atto 550
2220
1844
1762
1274
794
719
442
342
187
145
140
140
116
112
102
95
94
92
91
89
84
84
78
75
73
72
68
68
67
65
61
55
54
54
54
54
53
53
53
53
53
53
48
48
46
41
38
35
34
34
32
31
24
23
23
22
20
18
18
17
16
15
15
15
14
14
14
12
12
12
12
12
12
10
10
10
10
9
9
9
9
9
8
8
8
8
8
7
6
6
6
6
6
6
6
5
5
5
5
5
5
5
5
4
4
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Applikation
Immunoassay (IA)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen highly purified mouse IgG gamma 1, gamma 2a, gamma 2b and gamma 3 proteins
Isotyp IgG
Produktmerkmale Anti-Mouse IgG (gamma 1, 2a, 2b and 3 chain) conjugated to ATTO 550 is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
This product is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Reinigung This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. This product shows balanced reactivity to Mouse IgG1, IgG2a, IgG2b and IgG3 proteins and is suitable to screen IgG class hybridoma clones. Minimal cross reactivity is observed against other Mouse immunoglobulin classes or light chain proteins.
Antigen
Applikations-hinweise Optimal working dilutions should be determined experimentally by the investigator.
Kommentare

The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 500 µL
Konzentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handhabung Avoid cycles of freezing and thawing.
This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
Product is photosensitive and should be protected from light.
Lagerung -20 °C
Informationen zur Lagerung Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. This product is stable for several weeks at 0 °C as an undiluted liquid.
Haltbarkeit 12 months
Bilder des Herstellers
 image for Rabbit anti-Mouse (Murine) IgG (Chain gamma) antibody (Atto 550) (ABIN1043989) Rabbit anti-Mouse (Murine) IgG (Chain gamma) antibody (Atto 550)
 image for Rabbit anti-Mouse (Murine) IgG (Chain gamma) antibody (Atto 550) (ABIN1043989) Rabbit anti-Mouse (Murine) IgG (Chain gamma) antibody (Atto 550) (Image 2)
Western Blotting (WB) image for Rabbit anti-Mouse (Murine) IgG (Chain gamma) antibody (Atto 550) (ABIN1043989) Western Blot of ATTO 550 Rabbit Anti-Mouse IgG (gamma 1, 2a, 2b, 3) secondary antibod...