beta Catenin Antikörper (Catenin, beta) (C-Term)

Details for Product anti-Catenin, beta Antibody No. ABIN1043907, Anbieter: Anmelden zum Anzeigen
Antigen
  • Bfc
  • Catnb
  • Mesc
  • CTNNB
  • MRD19
  • armadillo
  • ctnnb
  • id:ibd2058
  • wu:fb73e10
  • wu:fi81c06
  • wu:fk25h01
  • beta-catenin
  • ctnnb1
  • CHBCAT
  • catenin (cadherin associated protein), beta 1
  • catenin beta 1
  • catenin (cadherin-associated protein), beta 1
  • catenin beta 1 L homeolog
  • Ctnnb1
  • CTNNB1
  • ctnnb1
  • ctnnb1.L
Epitop
C-Term
66
62
62
50
44
38
25
19
18
16
15
15
15
15
9
7
6
4
4
4
4
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Rind (Kuh), Human, Maus, Ratte (Rattus), Xenopus laevis, Zebrafisch (Danio rerio)
889
661
586
196
168
158
26
20
18
15
13
12
12
4
3
2
2
2
2
2
1
1
1
Wirt
Kaninchen
453
441
4
4
1
Klonalität
Polyklonal
Konjugat
Dieser beta Catenin Antikörper ist unkonjugiert
27
25
20
19
18
18
18
17
10
10
10
10
10
9
9
9
9
9
9
9
9
9
9
9
9
9
9
9
9
9
9
9
9
9
9
9
9
7
7
7
7
6
3
1
1
1
Applikation
ELISA, Western Blotting (WB)
710
441
396
324
227
189
126
120
56
21
13
13
8
5
5
2
2
1
1
1
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
'Independent Validation' Siegel
Chargennummer 27397
Validierte Anwendung Western Blotting
Positivkontrolle MCF-7 cells
Negativkontrolle SK-BR-3 cells
Bewertung A strong specific band was observed in the positive control at the expected size (~85.5 kDa) that is not observed in the negative control.
Primärantikörper
  • Antigen: Beta Catenin
  • Catalog number: ABIN1043907
  • Supplier: Anmelden zum Anzeigen
  • Lot number: 27397
  • Dilution: 1:1,000
Sekundärantikörper
Kontrollen
  • MCF-7 lysates were prepared by ADS Biosystems following standard protocols and quality controlled for protein integrity on a regular basis
  • SK-BR-3 lysates were purchased from Santa Cruz Biotechnology (catalog number sc-2218)
Protokoll
  • Lysates were mixed with NuPAGE® LDS Sample Buffer (Life Technologies NP0007) and NuPAGE® Sample Reducing Agent (Life Technologies NP0004) and denatured for 5 minutes at 90ºC.
  • 40 μg of each lysate was electrophoresed on a Bolt 4-12% Bis-Tris Gel (Life Technologies BG04120BOX) and run in Bolt MOPS SDS Running Buffer (Life Technologies B0001) at 160 volts for 1 hour.
  • Odyssey Western Protein Standard (LI-COR #928-40000) was run as a molecular weight standard.
  • PVDF membrane was activated with methanol.
  • Protein samples were transferred to activated PVDF membrane in a wet Bolt Transfer Apparatus (Life Technologies B1000) at room temperature for 1 hour at 20 volts (started at 230mA, ended at 110mA).
  • The membrane was blocked in x LI-COR Odyssey WB block solution for 1 hour at room temperature.
  • The membrane was incubated with the primary antibody diluted 1:1000 in x LI-COR Odyssey WB block solution incubated 2 hours at room temperature.
  • The membrane was washed 4 x 5 minutes in 1 x PBS-T (PBS solution with 0.1% Tween 20).
  • The membrane was incubated with IRDye® 800CW Goat anti-Mouse Secondary Antibody (Red) and IRDye 680LT Goat Anti-Rabbit Secondary Antibody (Green) from LI-COR (#827-11081, Lot #C30725-01), both 1:10,000 dilutions. Incubation was performed at room temperature for 45 minutes.
  • The membrane was washed 4 x 5 minutes in 1 x PBS-T (PBS solution with 0.1% Tween 20).
  • Proteins were detected using Odyssey machine scanning with green channel for loading control and red channel for potential LPL band.
Anmerkungen - No experimental challenges noted.
Validierungsbilder
Western Blotting validation image for anti-Catenin, beta (C-Term) antibody (ABIN1043907) Figure 1: Scanned image of Beta Catenin (Green) and loading control alpha-tubulin (Re...
Immunogen beta catenin antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to catenin beta-1 C-terminus.
Immunogen Type: Peptide
Isotyp IgG
Spezifität Beta catenin antibody is directed against catenin beta-1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross-reactivity with catenin beta-1 protein from human, mouse, rat, bovine, Xenopus laevis, and Danio rerio at 100% based on homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
Produktmerkmale Beta-catenin 1 (or ß-catenin 1) is a protein that is encoded by the CTNNB1 gene. ß-catenin 1 is a subunit of the cadherin protein complex and has been implicated as an integral component in the Wnt signaling pathway. This pathway plays a key role in the regulation of cellular processes involved in development, differentiation, and adult tissue homeostasis. In the presence of Wnt ligand, ß-catenin 1 is not ubiquitinated and accumulates in the nucleus, where it associates with T-cell factor (TCF) family members to regulate target gene expression in many developmental and adult tissues. Recruitment of b-catenin 1 to Wnt response element (WRE) chromatin converts TCFs from transcriptional repressors to activators. ß-catenin 1 is also involved in the regulation of cell adhesion. It acts as a negative regulator of centrosome cohesion. Aberrant Wnt/ß-catenin signaling is widely implicated in cancer, bone disorders, kidney and intestinal cell disorders and other disease states. ß-catenin 1 is located in the cytoplasm when it is unstabilized or bound to CDH1. Interaction with GLIS2 and MUC1 promotes nuclear translocation. Interaction with EMD inhibits nuclear localization. The majority of ß-catenin 1 is localized to the cell membrane. In interphase, colocalizes with CROCC between CEP250 puncta at the proximal end of centrioles, and this localization is dependent on CROCC and CEP250. In mitosis, when NEK2 activity increases, it localizes to centrosomes at spindle poles independent of CROCC. It further co-localizes with CDK5 in the cell-cell contacts and plasma membrane of undifferentiated and differentiated neuroblastoma cells.
Sterilität Sterile filtered
Plasmids, Primers & others Plasmide, Primers & weitere beta Catenin products on genomics-online (e.g. as negative or positive controls)
Antigen Catenin, beta
Andere Bezeichnung beta Catenin
Hintergrund Beta-catenin 1 (or ß-catenin 1) is a protein that is encoded by the CTNNB1 gene. ß-catenin 1 is a subunit of the cadherin protein complex and has been implicated as an integral component in the Wnt signaling pathway. This pathway plays a key role in the regulation of cellular processes involved in development, differentiation, and adult tissue homeostasis. In the presence of Wnt ligand, ß-catenin 1 is not ubiquitinated and accumulates in the nucleus, where it associates with T-cell factor (TCF) family members to regulate target gene expression in many developmental and adult tissues. Recruitment of b-catenin 1 to Wnt response element (WRE) chromatin converts TCFs from transcriptional repressors to activators. ß-catenin 1 is also involved in the regulation of cell adhesion. It acts as a negative regulator of centrosome cohesion. Aberrant Wnt/ß-catenin signaling is widely implicated in cancer, bone disorders, kidney and intestinal cell disorders and other disease states. ß-catenin 1 is located in the cytoplasm when it is unstabilized or bound to CDH1. Interaction with GLIS2 and MUC1 promotes nuclear translocation. Interaction with EMD inhibits nuclear localization. The majority of ß-catenin 1 is localized to the cell membrane. In interphase, colocalizes with CROCC between CEP250 puncta at the proximal end of centrioles, and this localization is dependent on CROCC and CEP250. In mitosis, when NEK2 activity increases, it localizes to centrosomes at spindle poles independent of CROCC. It further co-localizes with CDK5 in the cell-cell contacts and plasma membrane of undifferentiated and differentiated neuroblastoma cells.
Synonyms: Catenin beta-1, beta catenin, ß-catenin-1, ß-catenin, CTNNB1, CTNNB, beta catenin antibody, anti-beta catenin
NCBI Accession NP_001091679
UniProt Q9WU82
Pathways Peptide Hormone Metabolism
Applikations-hinweise beta catenin antibody has been tested for use in ELISA, western blotting, and ISH. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 85.5 kDa in size corresponding to catenin beta-1 protein by western blotting in the appropriate cell lysate or extract.
Kommentare

Gene Name: ctnnb1

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1.15 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
Haltbarkeit 12 months
Bilder des Herstellers
Western Blotting (WB) image for anti-Catenin, beta (C-Term) antibody (ABIN1043907) Western Blot of Rabbit anti-Beta Catenin antibody. Marker: Opal Pre-stained ladder . ...
Western Blotting (WB) image for anti-Catenin, beta (C-Term) antibody (ABIN1043907) Western Blot of Rabbit anti-catenin ß-1 antibody Lane 1: zebrafish embryos injected w...
Immunohistochemistry (IHC) image for anti-Catenin, beta (C-Term) antibody (ABIN1043907) Immunohistochemistry of Rabbit anti-beta Catenin antibody. Tissue: human small intest...
Produkt verwendet in: Mo, Chew, Maher, Bellipanni, Weinberg, Gottardi: "The terminal region of beta-catenin promotes stability by shielding the Armadillo repeats from the axin-scaffold destruction complex." in: The Journal of biological chemistry, Vol. 284, Issue 41, pp. 28222-31, 2009 (PubMed).

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