DLD Antikörper

Produktnummer ABIN1607742

Produktdetails anti-DLD Antikörper

Target: DLD (Dihydrolipoamide Dehydrogenase (DLD))

Reaktivität: Schwein

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DLD Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Kreuzreaktivität (Details): Cross reactivity against Lipoamide Dehydrogenase from other tissues and species may occur but have not been specifically determined.

Reinheit: Anti-LIPOAMIDE DEHYDROGENASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Lipoamide Dehydrogenase [Porcine Heart].

Endotoxin-Niveau: Low Endotoxin : No

Immunogen: Lipoamide Dehydrogenase [Porcine Heart]
Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Anti-LIPOAMIDE DEHYDROGENASE has been assayed against 1.0 µg of Lipoamide Dehydrogenase [Porcine Heart] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:7.000 to 1:30.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:20.000
IF Immunoprecipitation Dilution: 1:100
Western Blot Dilution: 1:500 - 1:5.000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Handhabung: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Haltbarkeit: Expiration date is one (1) year from date of opening.

Details zu DLD

Target: DLD (Dihydrolipoamide Dehydrogenase (DLD))

Andere Bezeichnung: Lipoamide Dehydrogenase (DLD Produkte)

Synonyme: DLDD antikoerper, DLDH antikoerper, E3 antikoerper, GCSL antikoerper, LAD antikoerper, PHE3 antikoerper, AI315664 antikoerper, AI746344 antikoerper, wu:fb24b05 antikoerper, DLD antikoerper, DDBDRAFT_0183800 antikoerper, DDBDRAFT_0216232 antikoerper, DDB_0183800 antikoerper, DDB_0216232 antikoerper, sc:d0402 antikoerper, dihydrolipoamide dehydrogenase antikoerper, dihydrolipoyl dehydrogenase antikoerper, deltaD antikoerper, DLD antikoerper, Dld antikoerper, dldh antikoerper, AT4G16155 antikoerper, CND05840 antikoerper, bfmBC antikoerper, GCSL antikoerper, LACBIDRAFT_182385 antikoerper, UREG_06178 antikoerper, lpd antikoerper, TAGG_RS02070 antikoerper, Arnit_2606 antikoerper, Mesil_1945 antikoerper, Trad_2118 antikoerper, Acear_0640 antikoerper, Fbal_0372 antikoerper, Ilyop_1890 antikoerper, Ftrac_1733 antikoerper, Ocepr_1753 antikoerper, Intca_2017 antikoerper, Deima_0504 antikoerper, dld antikoerper

Hintergrund: Synonyms: Dihydrolipoyl dehydrogenase, mitochondrial EC=1.8.1.4 Dihydrolipoamide dehydrogenase Lipoamide Dehydrogenase [Porcine Heart]

Gen-ID: 397129, 118675

UniProt: P09623

Pathways: Ribonucleoside Biosynthetic Process, Cell RedoxHomeostasis