Ziege anti-Meerschweinchen IgG (Heavy & Light Chain) Antikörper (Alkaline Phosphatase (AP)) - Preadsorbed

Produktnummer ABIN101273

Produktdetails

Target: IgG

Bindungsspezifität: Heavy & Light Chain

Reaktivität: Meerschweinchen

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Alkaline Phosphatase (AP)

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Spezifität: IgG (H&L)

Kreuzreaktivität: Meerschweinchen

Produktmerkmale: Anti-Guinea Pig IgG (H&L) antibody generated in goat detects specifically guinea pig IgG (H&L). This secondary antibody anti-Guinea Pig is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Concentration Definition: by UV absorbance at 280 nm

Aufreinigung: Preadsorption: Solid phase absorption

Sterilität: Sterile filtered

Immunogen:

Immunogen: Anti-Guinea Pig IgG (H&L) was produced by repeated immunization with Guinea Pig IgG whole molecule in goat.

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:200 - 1:1,000

Application Note: Antibody Anti-Guinea Pig IgG (H&L) is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry assays requiring lot-to-lot consistency.

ELISA Dilution: 1:2,000 - 1:10,000

Western Blot Dilution: 1:500 - 1:2,500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

Handhabung: Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed.

Lagerung: 4 °C

Haltbarkeit: 12 months

Antigendetails

Target: IgG

Abstract: IgG Produkte

Substanzklasse: Antibody

Hintergrund:

Synonyms: goat Anti-Guinea Pig IgG Antibody alkaline phosphatase Conjugation, goat Anti-Guinea Pig IgG alk phos Conjugated antibody

Background: Anti-Guinea Pig IgG Alkaline Phosphatase Antibody generated in goat detects guinea pig IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.