General
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Antigen
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Kininogen 1 (KNG1) Antikörper
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Reaktivität
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Human
Alternativen
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Wirt
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Ziege
Alternativen
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Klonalität
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Konjugat
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Unkonjugiert
Alternativen
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Applikation
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Immunodiffusion (ID), Immunoprecipitation (IP)
Alternativen
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Hersteller
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Anmelden zum Anzeigen
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Spezifität
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The defined antibody specificity is to Human High Molecular Weight Kininogen. In Immunoelectrophoresis, bi-dimensional Electrophoresis and Radial Immunodiffusion (Ouchterlony, Mancini), using various antiserum concentrations against normal plasma, one single precipitin line is obtained. No precipitation is observed against plasma of patients with high molecular weight kininogen deficiency, but its presence can be demonstrated in normal serum. In Double Radial Immunodiffusion the precipitin line obtained against normal plasma shows a reaction of full identity with the precipitin line with purified high molecular weight kininogen. The antiserum does not cross-react with any other human plasma proteins as tested in gel-diffusion techniques. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail, however in Double Radial Immunodiffusion a reaction with Monkey has been observed.
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Reinigung
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Serum
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Immunogen
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Highly purified Molecular Weight Kininogen isolated from pooled plasma. Freund’s complete adjuvant is used in the first step of the immunization procedure.
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Antigen
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Andere Bezeichnung
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Kininogen-1 (KNG1 Antibody Abstract)
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Hintergrund
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Synonyms: Alpha-2-thiol proteinase inhibitor, BDK, Fitzgerald factor, High molecular weight kininogen, KNG, KNG1, Williams-Fitzgerald-Flaujeac factor
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Gen-ID
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3827
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NCBI Accession
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NP_000884
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UniProt
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P01042
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Pathways
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ACE Inhibitor Pathway, Glycosaminoglycan Metabolic Process
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Applikations-hinweise
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Optimal working dilution should be determined by the investigator.
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Beschränkungen
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Nur für Forschungszwecke einsetzbar
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Format
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Liquid
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Rekonstitution
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Restore by adding 1 mL of sterile distilled water.
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Handhabung
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Avoid Repeated thawing and freezing.
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Lagerung
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4 °C/-20 °C
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Informationen zur Lagerung
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Store lyophilized at 2-8 °C and reconstituted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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