anti-SUMO1 Activating Enzyme Subunit 1 (SAE1) Antikörper

Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1\; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. Zusätzlich bieten wir Ihnen SAE1 Proteine (19) und SAE1 Kits (10) und viele weitere Produktgruppen zu diesem Protein an.

Alle Antikörper anzeigen Gen GeneID UniProt
SAE1 10055 Q9UBE0
SAE1 56459 Q9R1T2
SAE1 308384 Q6AXQ0
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Top anti-SAE1 Antikörper auf antikoerper-online.de

Showing 10 out of 140 products:

Katalog Nr. Reaktivität Wirt Konjugat Applikation Bilder Menge Anbieter Lieferzeit Preis Details
Rind (Kuh) Kaninchen Unkonjugiert WB <b>WB Suggested Anti-SAE1 Antibody Titration: </b>0.2-1 ug/ml<br><b>ELISA Titer: </b>1:1562500<br><b>Positive Control: </b>Hela cell lysate WB Suggested Anti-SAE1 <br /> Antibody Titration: 0.2-1 µg/mL ELISA Titer: 1:1562500 <br /> Positive Control: Hela cell lysate <br /> SAE1 is supported by BioGPS gene expression data to be expressed in HeLa 100 μL Anmelden zum Anzeigen 2 bis 3 Tage
$289.00
Details
Rind (Kuh) Kaninchen Unkonjugiert WB <b>WB Suggested Anti-SAE1 Antibody Titration: </b>0.2-1 ug/ml<br><b>ELISA Titer: </b>1:312500<br><b>Positive Control: </b>Human brain 100 μL Anmelden zum Anzeigen 2 bis 3 Tage
$289.00
Details
Human Kaninchen Unkonjugiert IC, IF, IHC, WB Immunofluorescent analysis of SAE1 staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Immunohistochemical analysis of SAE1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. 200 μL Anmelden zum Anzeigen 13 bis 14 Tage
$487.50
Details
Human Ziege Unkonjugiert ELISA, WB ABIN185082 staining (1µg/ml) of Jurkat lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. 100 μg Anmelden zum Anzeigen 6 bis 7 Tage
$358.02
Details
Human Kaninchen Unkonjugiert IHC (p), WB The AOS1 C-term Pab (ABIN389081) is used in Western blot to detect AOS1 in mouse heart tissue lysate. Western blot analysis of AOS1 (arrow) using rabbit polyclonal AOS1 Antibody (C-term) (ABIN389081). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the AOS1 gene (Lane 2) (Origene Technologies). 400 μL Anmelden zum Anzeigen 10 bis 11 Tage
$324.50
Details
Human Maus Unkonjugiert IF, WB Immunofluorescence of monoclonal antibody to SAE1 on HeLa cell (antibody concentration 10 µg/mL). SAE1 monoclonal antibody (M01), clone 1G4-1G5 Western Blot analysis of SAE1 expression in HeLa. 0.1 mg Anmelden zum Anzeigen 8 bis 11 Tage
$450.00
Details
Human Kaninchen Unkonjugiert ELISA, WB Western blot using Rabbit anti-SAE1 pS185 antibody shows detection of phosphorylated SAE1. Left lane (-) contains 20 µg human HeLa whole cell protein. Right lane (+) contains 20 µg human HeLa whole cell protein from cells pre-treated with phosphatase inhibitor cocktail to prevent dephos-phorylation of the target. Proteins were separated on a 10% SDS-PAGE and transferred onto nitrocellulose. After blocking with 5% milk-TBST 1 hr at room temperature, the membrane was probed with the primary antibody diluted to 1:1,000 at room temperature for 3 hr followed by washes and reaction with HRP-conjugated secondary and ECL imaging. Western blot using  Rabbit anti-SAE1 pS185 antibody shows detection of phosphorylated SAE1. Left lane  contains 20 µg human HeLa whole cell protein. Right lane (+) contains 20 µg human HeLa whole cell protein from cells pre-treated with phosphatase inhibitor cocktail to prevent dephos-phorylation of the target. Proteins were separated on a 10% SDS-PAGE and transferred onto nitrocellulose. After blocking with 5% milk-TBST 1 hr at room temperature, the membrane was probed with the primary antibody diluted to 1:1,000 at room temperature for 3 hr followed by washes and reaction with HRP-conjugated secondary and ECL imaging. Personal communication, Xin-Hua Feng, Baylor College of Medicine, Houstin, TX 100 μg Anmelden zum Anzeigen 1 bis 2 Tage
$389.00
Details
Human Kaninchen Unkonjugiert ELISA, WB Western blot using Rabbit anti-SAE1 antibody shows detection of SAE1. Left lane (-) contains 20 µg human HeLa whole cell protein. Right lane (+) contains 20 µg human HeLa whole cell protein from cells pre-treated with phosphatase inhibitor cocktail to prevent dephosphorylation of the target. Proteins were separated on a 10% SDS-PAGE and transferred onto nitrocellulose. After blocking with 5% milk-TBST 1 hr at room temperature, the membrane was probed with the primary antibody, Anti-SAE1, diluted to 2 µg/mL at room temperature for 3 hr followed by washes and reaction with HRP-conjugated secondary and ECL imaging. Western blot using  Rabbit anti-SAE1 antibody shows detection of SAE1. Left lane  contains 20 µg human HeLa whole cell protein. Right lane (+) contains 20 µg human HeLa whole cell protein from cells pre-treated with phosphatase inhibitor cocktail to prevent dephosphorylation of the target. Proteins were separated on a 10% SDS-PAGE and transferred onto nitrocellulose. After blocking with 5% milk-TBST 1 hr at room temperature, the membrane was probed with the primary antibody, Anti-SAE1, diluted to 2 µg/mL at room temperature for 3 hr followed by washes and reaction with HRP-conjugated secondary and ECL imaging. Personal communication, Xin-Hua Feng, Baylor College of Medicine, Houstin, TX. 100 μg Anmelden zum Anzeigen 1 bis 2 Tage
$389.00
Details
Schimpanse Kaninchen Unkonjugiert EIA, IF, IHC (p), WB 0.5 mg Anmelden zum Anzeigen 6 bis 8 Tage
$610.50
Details
Human Kaninchen Unkonjugiert IF, ELISA, WB Western blot analysis of extracts from 293 cells, using AOS1 Antibody. The lane on the right is treated with the synthesized peptide. Immunofluorescence analysis of HuvEc cells, using AOS1 Antibody. The picture on the right is treated with the synthesized peptide. 100 μg Anmelden zum Anzeigen 2 bis 3 Tage
$302.50
Details

SAE1 Antikörper nach Reaktivität, Anwendung, Klonalität and Konjugat

Attribute Applikationen Wirt Klonalität Konjugat
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Am meisten referenzierte anti-SAE1 Antikörper

  1. Human Monoclonal SAE1 Primary Antibody für IF, IHC (p) - ABIN564342 : Fujimoto, Matsushita, Hamaguchi, Kaji, Asano, Ogawa, Yamaoka, Fujikawa, Tsukada, Sato, Echigo, Hasegawa, Takehara: Autoantibodies to small ubiquitin-like modifier activating enzymes in Japanese patients with dermatomyositis: comparison with a UK Caucasian cohort. in Annals of the rheumatic diseases 2012 (PubMed)

  2. Human Polyclonal SAE1 Primary Antibody für ELISA, WB - ABIN250021 : Desterro, Rodriguez, Kemp, Hay: Identification of the enzyme required for activation of the small ubiquitin-like protein SUMO-1. in The Journal of biological chemistry 1999 (PubMed)

Weitere Antikörper gegen SAE1 Interaktionspartner

Human SUMO1 Activating Enzyme Subunit 1 (SAE1) Interaktionspartner

Zebrafish SUMO1 Activating Enzyme Subunit 1 (SAE1) Interaktionspartner

SAE1 Antigen-Profil

Protein Überblick

Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1\; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 and UBA2 (MIM 613295) form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999

Genbezeichner und Symbole assoziert mit SAE1

  • SUMO1 activating enzyme subunit 1 (SAE1) Antikörper
  • SUMO1 activating enzyme subunit 1 (Sae1) Antikörper
  • SUMO1 activating enzyme subunit 1 L homeolog (sae1.L) Antikörper
  • SUMO1 activating enzyme subunit 1 (sae1) Antikörper
  • 2400010M20Rik Antikörper
  • 2610044L12Rik Antikörper
  • AL033372 Antikörper
  • aos Antikörper
  • AOS1 Antikörper
  • Aos1p Antikörper
  • AW743391 Antikörper
  • D7Ertd177e Antikörper
  • HSPC140 Antikörper
  • sae2a Antikörper
  • Sua1 Antikörper
  • Sua1p Antikörper
  • uble1a Antikörper
  • wu:fa28b04 Antikörper
  • zgc:86633 Antikörper

Bezeichner auf Proteinebene für SAE1

SUMO-1 activating enzyme E1 N subunit , SUMO-1 activating enzyme subunit 1 , SUMO-activating enzyme subunit 1 , activator of SUMO1 , sentrin/SUMO-activating protein AOS1 , ubiquitin-like 1-activating enzyme E1A , ubiquitin-like protein SUMO-1 activating enzyme , ubiquitin-like (sentrin) activating enzyme E1A , ubiquitin-like 1 (sentrin) activating enzyme E1A , ubiquitin-like 1 (sentrin) activating enzyme subunit 1 , SUMO-activating enzyme E1 N subunit , SUMO1 activating enzyme subunit 2a , Ubiquitin-like 1-activating enzyme E1A , fa28b04

GENE ID SPEZIES
10055 Homo sapiens
56459 Mus musculus
308384 Rattus norvegicus
476425 Canis lupus familiaris
505512 Bos taurus
100173973 Pongo abelii
443558 Xenopus laevis
100718649 Cavia porcellus
415148 Danio rerio
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