Sclerostin (SOST) (AA 24-213) (Active) protein (Biotin,His tag)

Details zu Produkt Nr. ABIN2181783, Anbieter: Anmelden zum Anzeigen
Proteinname
  • LOC100313724
  • CDD
  • VBCH
  • 5430411E23Rik
  • sclerostin
  • LOC100313724
  • SOST
  • Sost
Proteineigenschaft
AA 24-213
6
1
1
1
1
1
1
1
Spezies
Human
10
3
2
1
1
Quelle
HEK-293 Cells
5
3
2
2
2
2
1
Protein-Typ
Recombinant
Biologische Aktivität
Active
Aufreinigungstag / Konjugat
Dieses Sclerostin Protein ist gelabelt mit Biotin,His tag.
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Hersteller
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Hersteller Produkt- Nr.
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Marke MABSol®,UltraLys
Spezifität The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.
Produktmerkmale This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 22.5 kDa. The protein migrates as 25-36 kDa on a SDS-PAGE gel under reducing (R) condition due to different glycosylation.
Reinheit >95 % as determined by reduced SDS-PAGE.
Sterilität 0.22 μm filtered
Endotoxin-Niveau Less than 1.0 EU per μg by the LAL method.
Güteklasse Ultra Sensitive
Hintergrund Sclerostin (SOST) also known as Sclerosteosis, VBCH, is a secreted glycoprotein with a signal peptide for secretion and a C-terminal cysteine knot-like (CTCK) domain and belongs to the Cerberus/DAN family of bone morphogenetic protein (BMP) antagonists. Sclerostin is produced by the osteocyte and has anti-anabolic effects on bone formation. More recently Sclerostin has been identified as binding to LRP5/6 receptors and inhibiting the Wnt signalling pathway. Wnt pathway inhibition under these circumstances is antagonistic to bone formation (meaning Sclerostin antagonizes bone formation). It has been shown that SOST binds BMP-5, -6, and -7 with high affinity and BMP-2 and -4 with low affinity. Sclerostin production by osteocytes is inhibited by parathyroid hormone, mechanical loading and cytokines including oncostatin M, cardiotrophin-1 and leukemia inhibitory factor. Sclerostin production is increased by calcitonin. Thus, osteoblast activity is self regulated by a negative feedback system. Mutations of Sclerostin is associated with the syndrome Sclerosteosis, and reduced sclerostin expression results in a milder form of the disorder called van Buchem disease.
Molekulargewicht 22.5 kDa
UniProt Q9BQB4
Kommentare

A chemically labeled biotinylated protein with ultra sensitivity.
The product is produced using a chemical labeling approach. The primary amines in the side chains of lysine residues and the N-terminus of protein are conjugated with biotins.
Chemical labeling usually results in multiple biotin attachment on a single protein molecule, which could potentially lead to higher detection sensitivity.

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Buffer PBS, pH 7.4
Handhabung Avoid repeated freeze-thaw cycles.
Lagerung -20 °C
Informationen zur Lagerung No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
Bilder des Herstellers
SDS-PAGE (SDS) image for Sclerostin (SOST) (AA 24-213) (Active) protein (Biotin,His tag) (ABIN2181783) Biotinylated Human SOST on SDS-PAGE under reducing (R) condition. The gel was stained...
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