anti-Human PIP5K1A Antikörper für Enzyme Immunoassay

Recommended PIP5K1A Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Phosphatidylinositol-4-Phosphate 5-Kinase, Type I, alpha (PIP5K1A) Antikörper
  • Pip5k1b
  • pip5k1c
  • PIP5K1A
  • pip5k1b
  • Pipk5a
  • Pipk5b
  • phosphatidylinositol-4-phosphate 5-kinase, type 1, alpha
  • phosphatidylinositol-4-phosphate 5-kinase, type I, alpha
  • phosphatidylinositol-4-phosphate 5-kinase, type 1 alpha
  • Pip5k1a
  • pip5k1a
  • PIP5K1A
Epitop
N-Term
18
4
2
2
1
1
1
1
1
1
Reaktivität
Human
37
11
10
2
2
2
2
2
2
1
1
1
1
1
1
Wirt
Kaninchen
36
1
Klonalität
Polyklonal
Konjugat
Dieser PIP5K1A Antikörper ist unkonjugiert
2
2
2
2
2
2
Applikation
Enzyme Immunoassay (EIA), Western Blotting (WB)
36
22
7
6
4
3
2
2
Hersteller
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Hersteller Produkt- Nr.
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Bilder

Produktnummer ABIN360478
$ 390.50
Zzgl. Versandkosten $45.00

General

Antigen Phosphatidylinositol-4-Phosphate 5-Kinase, Type I, alpha (PIP5K1A) Antikörper
Epitop N-Term
(18), (4), (2), (2), (1), (1), (1), (1), (1), (1)
Reaktivität Human
(37), (11), (10), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Wirt Kaninchen
(36), (1)
Klonalität
Polyklonal
Konjugat Dieser PIP5K1A Antikörper ist unkonjugiert
(2), (2), (2), (2), (2), (2)
Applikation Enzyme Immunoassay (EIA), Western Blotting (WB)
(36), (22), (7), (6), (4), (3), (2), (2)
Pubmed 3 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-PIP5K1A Antikörper

Target Details PIP5K1A Anwendungsinformationen Handhabung Referenzen für anti-PIP5K1A Antikörper (ABIN360478) Bilder
Spezifität This antibody reacts to PIP5K1A.
Reinigung Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human PIP5K1A.
Isotyp Ig

Target Details PIP5K1A

Produktdetails anti-PIP5K1A Antikörper Anwendungsinformationen Handhabung Referenzen für anti-PIP5K1A Antikörper (ABIN360478) Bilder zurück nach oben
Antigen
Andere Bezeichnung PIP5K1A (PIP5K1A Antibody Abstract)
Hintergrund Overexpression of phosphatidylinositol phosphate 5-kinase alpha (PIP5KIalpha), which synthesizes PIP2, suppresses apoptosis, whereas a kinase-deficient mutant does not. Protection by the wild-type PIP5KIalpha isaccompanied by decreases in the generation of activated caspases and of caspase 3-cleaved PARP. Protection is not mediated through PIP3 or Akt activation. An anti-apoptotic role for PIP(2) is substantiated by the finding that PIP5KIalpha is cleaved by caspase 3 during apoptosis, and cleavage inactivates PIP5KIalpha in vitro. Mutation of the P(4) position (D279A) of the PIP5KIalpha caspase 3 cleavage consensus prevents cleavage in vitro, and during apoptosis in vivo. Significantly, the caspase 3-resistant PIP5KIalpha mutant is more effective in suppressing apoptosis than the wild-type kinase. PIP2 is a direct regulator of apical and effector caspases in the death receptor and mitochondrial pathways, and PIP5KIalpha inactivation contributes to the progression of apoptosis.Synonyms: 68 kDa type I phosphatidylinositol-4-phosphate 5-kinase alpha, PIP5K1-alpha, PIP5KIalpha, Phosphatidylinositol-4-phosphate 5-kinase type-1 alpha, PtdIns(4)P-5-kinase 1 alpha
Gen-ID 8394, 9606
UniProt Q99755
Pathways PI3K-Akt Signalweg, Mitotic G1-G1/S Phases, Inositol Metabolic Process, DNA Replication, Cell-Cell Junction Organization, Synthesis of DNA

Anwendungsinformationen

Produktdetails anti-PIP5K1A Antikörper Target Details PIP5K1A Handhabung Referenzen für anti-PIP5K1A Antikörper (ABIN360478) Bilder zurück nach oben
Applikationshinweise ELISA: 1/1,000. Western blotting: 1/100 - 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-PIP5K1A Antikörper Target Details PIP5K1A Anwendungsinformationen Referenzen für anti-PIP5K1A Antikörper (ABIN360478) Bilder zurück nach oben
Format Liquid
Konzentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid repeated freezing and thawing.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Referenzen für anti-PIP5K1A Antikörper (ABIN360478)

Produktdetails anti-PIP5K1A Antikörper Target Details PIP5K1A Anwendungsinformationen Handhabung Bilder zurück nach oben
Allgemeine Veröffentlichungen

Doughman, Firestone, Wojtasiak, Bunce, Anderson: "Membrane ruffling requires coordination between type Ialpha phosphatidylinositol phosphate kinase and Rac signaling." in: The Journal of biological chemistry, Vol. 278, Issue 25, pp. 23036-45, 2003

Xie, Zhu, Zhao: "Assignment of type I phosphatidylinositol-4-phosphate 5-kinase (PIP5K1A) to human chromosome bands 1q22--> q24 by in situ hybridization." in: Cytogenetics and cell genetics, Vol. 88, Issue 3-4, pp. 197-9, 2000

Loijens, Anderson: "Type I phosphatidylinositol-4-phosphate 5-kinases are distinct members of this novel lipid kinase family." in: The Journal of biological chemistry, Vol. 271, Issue 51, pp. 32937-43, 1997

Bilder

Produktdetails anti-PIP5K1A Antikörper Target Details PIP5K1A Anwendungsinformationen Handhabung Referenzen für anti-PIP5K1A Antikörper (ABIN360478) zurück nach oben
Bilder des Herstellers
 image for anti-Phosphatidylinositol-4-Phosphate 5-Kinase, Type I, alpha (PIP5K1A) (N-Term) antibody (ABIN360478) anti-Phosphatidylinositol-4-Phosphate 5-Kinase, Type I, alpha (PIP5K1A) (N-Term) antibody