IL12 ELISA Kit

Produktnummer ABIN2690561

Produktdetails IL12 ELISA Kit

Target: IL12 (Interleukin 12 (IL12))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 7.8 - 500 pg/mL

Untere Nachweisgrenze: 7.8 pg/mL

Applikation: ELISA

Verwendungszweck: The human IL-12 (p70) ELISA Kit is for the in vitro quantitative determination of human IL-12 (p70) in serum, plasma, and cell culture supernatant.

Marke: BD OptEIA™

Proben: Serum, Plasma, Cell Culture Supernatant

Analytische Methode: Quantitative

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

BD OptEIA™ Human IL-12 (p70) ELISA Kit

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: 1. Store unopened kit at 2 - 8°C. Do not use kit after expiration date.
2. Before use, bring all reagents to room temperature (18 - 25°C). Immediately after use, return to proper storage conditions.
3. Lyophilized standards are stable until kit expiration date. After reconstitution, use freshly reconstituted standard within 12 hours (stored at 2 - 8°C).

Referenzen für IL12 ELISA Kit (ABIN2690561)

Ling, Gately, Gubler, Stern, Lin, Hollfelder, Su, Pan, Hakimi: "Human IL-12 p40 homodimer binds to the IL-12 receptor but does not mediate biologic activity." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 1, pp. 116-27, (1995) (PubMed).

Schoenhaut, Chua, Wolitzky, Quinn, Dwyer, McComas, Familletti, Gately, Gubler: "Cloning and expression of murine IL-12." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 148, Issue 11, pp. 3433-40, (1992) (PubMed).

Details zu IL12

Target: IL12 (Interleukin 12 (IL12))

Andere Bezeichnung: IL-12 (IL12 Produkte)

Synonyme: CLMF ELISA Kit, CLMF2 ELISA Kit, IL-12B ELISA Kit, NKSF ELISA Kit, NKSF2 ELISA Kit, p40 ELISA Kit, Il-12b ELISA Kit, Il12p40 ELISA Kit, Il-12p40 ELISA Kit, Il12 ELISA Kit, IL-12p40 ELISA Kit, IL-12 ELISA Kit, LOC100217488 ELISA Kit, IL-12p35 ELISA Kit, Il-12a ELISA Kit, Ll12a ELISA Kit, p35 ELISA Kit, IL12p40 ELISA Kit, IL12B ELISA Kit, IL-12 p35 ELISA Kit, interleukin 12B ELISA Kit, interleukin 12A ELISA Kit, interleukin 12b ELISA Kit, interleukin 12a ELISA Kit, Interleukin 12 level ELISA Kit, IL12B ELISA Kit, IL12A ELISA Kit, Il12b ELISA Kit, Il12a ELISA Kit, IL12 ELISA Kit, il12a ELISA Kit

Hintergrund: Human Interleukin-12 (p70) (hIL-12), also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a potent regulator of cell-mediated immune responses. Biologically active IL-12 is secreted by activated B lymphocytes and macrophages as a 70 kD heterodimeric glycoprotein composed of disulfide-bonded 35 kD (p35) and 40 kD (p40) subunits. The BD OptEIA™ kit specifically measures only this biologically active p70.
IL-12 stimulates the growth of activated CD4+ and CD8+ T cells and NK cells. IL-12 also promotes the development of proinflammatory-Th1-like CD4+ cells and cytotoxic CD8+ T cells. Other effects include increasing the lytic activity of NK cells and antibody-dependent cell-mediated cytotoxic cells, synergizing with IL-2 in the generation of lymphokine-activated killer (LAK) cells, and inducing NK and T cells to secrete large amounts of the proinflammatory cytokine, IFN-γ.

Pathways: JAK-STAT Signalweg, TLR Signalweg, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome