p65 Antikörper (Nuclear Factor-KB P65) (AA 50-100)

Details for Product anti-NFkBP65 Antibody No. ABIN668961, Anbieter: Anmelden zum Anzeigen
Antigen
  • NFKB3
  • p65
  • NFkB
  • v-rel avian reticuloendotheliosis viral oncogene homolog A
  • v-rel reticuloendotheliosis viral oncogene homolog A (avian)
  • RELA
  • Rela
Epitop
AA 50-100
146
84
68
63
51
49
44
41
40
37
33
26
26
24
18
17
15
15
15
13
13
13
12
11
10
9
9
8
6
6
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus, Schwein, Ratte (Rattus)
1245
681
521
72
59
55
31
30
28
26
15
8
8
6
5
4
2
1
1
Wirt
Kaninchen
1074
148
17
16
11
1
Klonalität
Polyklonal
Konjugat
Dieser p65 Antikörper ist unkonjugiert
34
34
26
21
19
18
16
16
13
13
13
9
9
9
9
9
9
8
8
4
4
3
3
3
2
2
2
2
2
2
1
Applikation
Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
912
459
359
268
237
159
117
92
83
28
22
19
18
12
11
6
6
6
6
5
4
2
2
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Immunogen KLH conjugated synthetic peptide derived from human NFKBp65
Isotyp IgG
Reinigung Purified by Protein A.
Andere Bezeichnung NFKB p65 (NFkBP65 Antibody Abstract)
Hintergrund

NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p15, NFKB1/p5, REL and NFKB2/p52 and the heterodimeric p65-p5 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p5 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1.

Subcellular location: Cytoplasm

Synonyms: p65, NFKB3, Transcription factor p65, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, RELA

Gen-ID 5970
UniProt Q04206
Pathways NF-kappaB Signalweg, RTK Signalweg, T-Zell Rezeptor Signalweg, TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades
Applikationshinweise WB 1:100-1000
FCM 1:20-100
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20°C
Haltbarkeit 12 months
Bilder des Herstellers
Immunohistochemistry (IHC) image for anti-Nuclear Factor-KB P65 (NFkBP65) (AA 50-100) antibody (ABIN668961) Formalin-fixed and paraffin embedded human endometrium carcinoma labeled with Rabbit ...
Immunohistochemistry (IHC) image for anti-Nuclear Factor-KB P65 (NFkBP65) (AA 50-100) antibody (ABIN668961) Formalin-fixed and paraffin-embedded rat aortic tissue labeled with Rabbit Anti-NFKB ...
Flow Cytometry (FACS) image for anti-Nuclear Factor-KB P65 (NFkBP65) (AA 50-100) antibody (ABIN668961) Mouse splenocytes probed with Rabbit Anti-NFkB p65 Polyclonal Antibody, Unconjugated .
Western Blotting (WB) image for anti-Nuclear Factor-KB P65 (NFkBP65) (AA 50-100) antibody (ABIN668961) HCCLM3 lysates probed with Anti-NFKB p65 Polyclonal Antibody, Unconjugated at 1:5000...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Nuclear Factor-KB P65 (NFkBP65) (AA 50-100) antibody (ABIN668961) Formalin-fixed and paraffin embedded rat spleen labeled with Rabbit Anti-NFKB p65 Pol...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Nuclear Factor-KB P65 (NFkBP65) (AA 50-100) antibody (ABIN668961) Formalin-fixed and paraffin embedded human endometrium carcinoma labeled with Rabbit ...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Nuclear Factor-KB P65 (NFkBP65) (AA 50-100) antibody (ABIN668961) Paraformaldehyde-fixed, paraffin embedded rat bladder, Antigen retrieval by boiling i...
Produkt verwendet in: Ganguly, Wen, Myer, Czech, Sahu, Steinmetz, Raman: "Anti-atherogenic effect of trivalent chromium-loaded CPMV nanoparticles in human aortic smooth muscle cells under hyperglycemic conditions in vitro." in: Nanoscale, Vol. 8, Issue 12, pp. 6542-54, 2016 (PubMed).

Yang, Zhong, Li, Xiong, Wang, Peng, Ye: "Hypothermic machine perfusion increases A20 expression which protects renal cells against ischemia/reperfusion injury by suppressing inflammation, apoptosis and necroptosis." in: International journal of molecular medicine, Vol. 38, Issue 1, pp. 161-71, 2016 (PubMed). Von den Autoren verwendete Methode: Western Blotting (WB) (Probematerial (Species): Rabbit).

Oyagbemi, Omobowale, Asenuga, Adejumobi, Ajibade, Ige, Ogunpolu, Adedapo, Yakubu: "Sodium fluoride induces hypertension and cardiac complications through generation of reactive oxygen species and activation of nuclear factor kappa beta." in: Environmental toxicology, 2016 (PubMed).

Xi, Hu, Guo, Wang, Sheng, Han, Yuan, Weng, Xu: "Immunoreactivities of NF-κB, IL-1β and IL-1R in the skin of Chinese brown frog (Rana dybowskii)." in: Acta histochemica, Vol. 119, Issue 1, pp. 64-70, 2016 (PubMed). Von den Autoren verwendete Methode: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Zheng, Liu, Li, Tang, Zhang, Tang: "Lithium posttreatment confers neuroprotection through glycogen synthase kinase-3β inhibition in intracerebral hemorrhage rats." in: Journal of neurosurgery, pp. 1-9, 2016 (PubMed). (Probematerial (Species): Rat (Rattus)).

Huang, Yang, Wang, Ma, Cui, Huang, Sheng, Weng, Xu: "Immunostimulatory activity of protein hydrolysate from oviductus ranae on macrophage in vitro." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2014, pp. 180234, 2015 (PubMed).

Luo, Yang, Tang, Yao, Kong, He, Zhou: "Kaempferol alleviates insulin resistance via hepatic IKK/NF-?B signal in type 2 diabetic rats." in: International immunopharmacology, Vol. 28, Issue 1, pp. 744-50, 2015 (PubMed).

Zhang, Wu, Song, Wan, Zhang, Tai, Liu: "Effect of hydrogen-rich water on acute peritonitis of rat models." in: International immunopharmacology, Vol. 21, Issue 1, pp. 94-101, 2014 (PubMed).

Niu, Mu, Li, Huang, Yao, Li: "Protective Effects of Chelerythrine Against Lipopolysaccharide-Induced Endotoxic Shock in Mice." in: Inflammation, 2014 (PubMed).

Wang, Ma, Su: "NF-?B pathway contributes to cadmium-induced apoptosis of porcine granulosa cells." in: Biological trace element research, Vol. 153, Issue 1-3, pp. 403-10, 2013 (PubMed). (1:100).

Song, Wang, Huang, Xiao, Shen, Xu: "NF ?B expression increases and CFTR and MUC1 expression decreases in the endometrium of infertile patients with hydrosalpinx: a comparative study." in: Reproductive biology and endocrinology : RB&E, Vol. 10, pp. 86, 2013 (PubMed).

Lin, Yan, Lin, Zhang, Wang, Yang, Zhang: "SIRT1 regulates TNF-α-induced expression of CD40 in 3T3-L1 adipocytes via NF-κB pathway." in: Cytokine, Vol. 60, Issue 2, pp. 447-55, 2012 (PubMed).

Zhao, Huang, Wang, Zhong: "Inhibition of NF-kappaB activation by Pyrrolidine dithiocarbamate partially attenuates hippocampal MMP-9 activation and improves cognitive deficits in streptozotocin-induced diabetic rats." in: Behavioural brain research, Vol. 238, pp. 44-7, 2012 (PubMed). Von den Autoren verwendete Methode: Western Blotting (WB) (Probematerial (Species): Rat (Rattus)).

Jiang, Tian, Fu, Zhu, Hou: "Neuroprotective efficacy and therapeutic window of Forsythoside B: in a rat model of cerebral ischemia and reperfusion injury." in: European journal of pharmacology, Vol. 640, Issue 1-3, pp. 75-81, 2010 (PubMed).

Jing, Piao, Liu, Gao: "Beta2-GPI: a novel factor in the development of hepatocellular carcinoma." in: Journal of cancer research and clinical oncology, Vol. 136, Issue 11, pp. 1671-80, 2010 (PubMed).

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