Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Aufreinigung
This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen
This Phospho-Caspase 9-S196 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S196 of human caspase 9.
CASP9
Reaktivität: Human, Maus, Ratte
WB, ELISA, IHC (p), FACS, IF (p), IF (cc), IHC (fro)
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
WB: 1:1000. IHC-P: 1:50~100
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Buffer
Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C,-20 °C
Informationen zur Lagerung
Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Haltbarkeit
6 months
Sangawa, Shintani, Yamao, Kamoshida: "Phosphorylation status of Akt and caspase-9 in gastric and colorectal carcinomas." in: International journal of clinical and experimental pathology, Vol. 7, Issue 6, pp. 3312-7, (2014) (PubMed).
Castells, Milhas, Gandy, Thibault, Rafii, Delord, Couderc: "Microenvironment mesenchymal cells protect ovarian cancer cell lines from apoptosis by inhibiting XIAP inactivation." in: Cell death & disease, Vol. 4, pp. e887, (2014) (PubMed).
Caspase 9 is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspase APAF1, this step is thought to be one of the earliest in the caspase activation cascade.