ATF2 Antikörper (C-Term)

Produktnummer ABIN374122

Produktdetails anti-ATF2 Antikörper

Target: ATF2 (Activating Transcription Factor 2 (ATF2))

Bindungsspezifität: C-Term

Reaktivität: Human, Ratte, Rind (Kuh), Hund

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser ATF2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Sequenz: C-IVMAPSSQSQ PSGS

Spezifität: This antibody detects ATF2 at C-term.

Kreuzreaktivität (Details): Species reactivity (expected):Rat, Canine (Dog), Bovine.
Species reactivity (tested):Human.

Aufreinigung: Ammonium Sulphate precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide from C-Terminus of the protein sequence according to NP_001871.2 Genename: ATF2

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Limit dilution 1: 32000. Western blot: Recommended concentration: 0.5-1.5 μg/mL. Experiments gave bands at

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 %

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu ATF2

Target: ATF2 (Activating Transcription Factor 2 (ATF2))

Andere Bezeichnung: ATF2 (ATF2 Produkte)

Synonyme: CRE-BP1 antikoerper, CREB2 antikoerper, HB16 antikoerper, TREB7 antikoerper, CG12850 antikoerper, CG3749 antikoerper, dATF-2 antikoerper, DmelCG30420 antikoerper, CG30420 antikoerper, CHN1 antikoerper, Atf-2 antikoerper, CRE-BP antikoerper, Creb2 antikoerper, D130078H02Rik antikoerper, D18875 antikoerper, Tg(Gzma-Klra1)7Wum antikoerper, mXBP antikoerper, atf2-A antikoerper, atf2l antikoerper, activating transcription factor 2 antikoerper, Activating transcription factor-2 antikoerper, activating transcription factor 2 S homeolog antikoerper, ATF2 antikoerper, Atf-2 antikoerper, Atf2 antikoerper, atf2.S antikoerper, atf2 antikoerper

Hintergrund: ATF2 (Activating Transcription Factor 2, CREBP, HB16, CREB2, TREB7) is a member of the ATF/CREB family of basic region leucine zipper DNA binding proteins that regulates transcription by binding to a consensus cAMP response element (CRE) in the promoter of various viral and cellular genes. Many of these genes are important in cell growth and differentiation, and in stress and immune responses. ATF2 is a nuclear protein that binds DNA as a dimer and can form dimers with members of the ATF/CREB and Jun/Fos families. It is a stronger activator as a heterodimer with cJun than as a homodimer. Several isoforms of ATF2 arise by differential splicing. The stable native full length ATF2 is transcriptionally inactive as a result of an inhibitory direct intramolecular interaction of its carboxy terminal DNA binding domain with the amino terminal transactivation domain. Following dimerization ATF2 becomes a short lived protein that undergoes ubiquitination and proteolysis, seemingly in a protein phosphatase-dependent mechanism. Stimulation of the transcriptional activity of ATF2 occurs following cellular stress induced by several genotoxic agents, inflammatory cytokines, and UV irradiation. This activation requires phosphorylation of two threonine residues in ATF2 by both JNK/SAP kinase and p38 MAP kinase. ATF2 is abundantly expressed in brain.Synonyms: ATF-2, Activating transcription factor 2, CRE-BP1, CREB-2, CREB2, CREBP1, Cyclic AMP-dependent transcription factor ATF-2, Cyclic AMP-responsive element-binding protein 2, HB16, cAMP response element-binding protein CRE-BP1, cAMP-dependent transcription factor ATF-2

Gen-ID: 1386

NCBI Accession: NP_001871

UniProt: P15336

Pathways: MAPK Signalweg, RTK Signalweg, Thyroid Hormone Synthesis, Activation of Innate immune Response, Chromatin Binding, Myometrial Relaxation and Contraction, Synaptic Membrane, Tube Formation, Toll-Like Receptors Cascades