p38 Antikörper (MAP kinase p38)

Details for Product anti-p38 Antibody No. ABIN361680, Anbieter: Anmelden zum Anzeigen
Antigen
  • CSBP
  • CSBP1
  • CSBP2
  • CSPB1
  • EXIP
  • Mxi2
  • PRKM14
  • PRKM15
  • RK
  • SAPK2A
  • p38
  • p38ALPHA
  • mitogen-activated protein kinase 14
  • MAPK14
Reaktivität
Human, Maus, Ratte (Rattus)
280
222
216
38
22
22
21
20
19
18
18
18
15
15
12
4
1
1
Wirt
Maus
198
84
Klonalität (Klon)
Monoklonal ()
Konjugat
Dieser p38 Antikörper ist unkonjugiert
7
7
7
7
6
5
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Applikation
Antibody Array (AA), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
223
160
109
68
61
56
48
28
27
17
13
10
6
6
2
2
1
1
1
1
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
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Immunogen Full length recombinant protein expressed in E.coli cells
Klon 9F12
Isotyp IgG1
Spezifität Detects ~38 kDa.
Reinigung Protein G Purified
Andere Bezeichnung p38 MAPK (p38 Antibody Abstract)
Hintergrund The MAPK (mitogen activated protein kinase) comprises a family of ubiquitous praline-directed, proteinserine/ threonine kinases which signal transduction pathways that control intracellular events including acute responses to hormones and major developmental changes in organisms (1). This super family consists of stress activated protein kinases (SAPKs), extracellular signal-regulated kinases (ERKs), and p38 kinases, each of which forms a separate pathway (2). The kinase members that populate each pathway are sequentially activated by phosphorylation. Upon activation, p38 MAPK/SAPK2α translocates into the nucleus where it phosphorylates one or more nuclear substrates, effecting transcriptional changes and other cellular processes involved in cell growth, division, differentiation, inflammation, and death (3). Specifically p38 always acts as a pro-apoptotic factor with its activation leading to the release of cytochrome c from mitochondria and cleavage of caspase 3 and its downstream effector, PARP (4). p38 MAPK is activated by a variety of chemical stress inducers including hydrogen peroxide, heavy metals, anisomycin, sodium salicylate, LPS, and biological stress signals such as tumor necrosis factor, interleukin-1, ionizing and UV irradiation, hyperosmotic stress and chemotherapeutic drugs (5). As a result, p38 alpha has been widely validated as a target for inflammatory disease including rheumatoid arthritis, COPD and psoriasis (6) and has also been implicated in cancer, CNS and diabetes (7).
Gen-ID 1432
NCBI Accession NP_001306
UniProt Q16539
Forschungsgebiet Kinases/Phosphatases
Pathways MAPK Signalweg, Neurotrophin Signalübertragung, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Muscle Cell Differentiation, Hepatitis C, Toll-Like Receptors Cascades, Autophagie, Thromboxane A2 Receptor Signaling
Applikationshinweise
  • WB (1:1000)
  • IHC (1:1000)
  • optimal dilutions for assays should be determined by the user.
Kommentare

Detects ~38kDa protein corresponding to p38α MAPK when loaded with 6 ng of purified p38α by chemiluminescent immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer PBS, 50 % glycerol, 0.09 % sodium azide
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Bilder des Herstellers
 image for anti-MAP kinase p38 (p38) antibody (ABIN361680) p38 MAPkinase (9F12), human cell lines.
Allgemeine Veröffentlichungen Nemes, Petrovski, Aerts, Sergeant, Devreese, Fuesues: "Transglutaminase-mediated intramolecular cross-linking of membrane-bound alpha-synuclein promotes amyloid formation in Lewy bodies." in: The Journal of biological chemistry, Vol. 284, Issue 40, pp. 27252-64, 2009 (PubMed).

Fan, Chen, Qiao, Luo, Ma, Li, Jiang, Niu, Yin: "Opposing effects of ERK and p38 MAP kinases on HeLa cell apoptosis induced by dipyrithione." in: Molecules and cells, Vol. 23, Issue 1, pp. 30-8, 2007 (PubMed).

Medicherla, Protter, Ma, Mangadu, Almirez, Koppelman, Kerr, Navas, Movius, Reddy, Liu, Luedtke, Perumattam, Mavunkel, Dugar, Schreiner: "Preventive and therapeutic potential of p38 alpha-selective mitogen-activated protein kinase inhibitor in nonobese diabetic mice with type 1 diabetes." in: The Journal of pharmacology and experimental therapeutics, Vol. 318, Issue 1, pp. 99-107, 2006 (PubMed).

Salojin, Owusu, Millerchip, Potter, Platt, Oravecz: "Essential role of MAPK phosphatase-1 in the negative control of innate immune responses." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 176, Issue 3, pp. 1899-907, 2006 (PubMed).

Van Laethem, Van Kelst, Lippens, Declercq, Vandenabeele, Janssens, Vandenheede, Garmyn, Agostinis: "Activation of p38 MAPK is required for Bax translocation to mitochondria, cytochrome c release and apoptosis induced by UVB irradiation in human keratinocytes." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 18, Issue 15, pp. 1946-8, 2004 (PubMed).

Deng, Ren, Yang, Lin, Wu: "A JNK-dependent pathway is required for TNFalpha-induced apoptosis." in: Cell, Vol. 115, Issue 1, pp. 61-70, 2003 (PubMed).

Pearson, Robinson, Beers Gibson, Xu, Karandikar, Berman, Cobb: "Mitogen-activated protein (MAP) kinase pathways: regulation and physiological functions." in: Endocrine reviews, Vol. 22, Issue 2, pp. 153-83, 2001 (PubMed).

Han, Lee, Bibbs, Ulevitch: "A MAP kinase targeted by endotoxin and hyperosmolarity in mammalian cells." in: Science (New York, N.Y.), Vol. 265, Issue 5173, pp. 808-11, 1994 (PubMed).

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