This antibody recognizes the 90 kDa MDM2 protein, which forms a complex with p53 leading to p53 inhibition. May also acts to stimulate cell proliferation via its induction of transcription factors such as E2F1 and DPI. MDM2 is over expressed in a range of human malignancies, including soft tissue sarcomas, breast cancer and bladder cancer. Cellular Localization: nuclear.
A Synthetic peptide corresponding to amino acids 154-167 of human MDM2 protein. Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63Ag8. 653myeloma cell line. AA Sequence: Cys-SRPSTSSRRRAISE
Western Blot: 1/1000. Immunoprecipitation. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections: 1/200-1/500. This product requires antigenretrieval using heat treatment prior to staining of paraffin sections. EDTA pH 8.0 isrecommended for this purpose. Recommended Positive Control: Breast Carcinoma. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
1.0 mg/mL
Buffer
PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Haltbarkeit
12 months
Target
MDM2
(Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2))
Human homolog of Murine Double Minute-2 (MDM2) and p53 function in mutual regulation: activation of p53 results in expression of MDM2 which consequently inhibits the ability of wild type p53 to stimulate expression of target genes. MDM2 is frequently overexpressed in the nuclei of soft tissue sarcomas, and to a much lesser extent, in a wide variety of malignancies of bladder, breast, testes, cervix, and glial cells.Synonyms: Double minute 2 protein, E3 ubiquitin-protein ligase Mdm2, Hdm2, Oncoprotein Mdm2, p53-binding protein Mdm2