Interleukin 6 Antikörper (IL6) (HRP)

Details for Product anti-IL6 Antibody No. ABIN1043799, Anbieter: Anmelden zum Anzeigen
Antigen
  • Il-6
  • ILg6
  • Ifnb2
  • BSF2
  • HGF
  • HSF
  • IFNB2
  • IL-6
  • CHIL-6
  • il6
  • interleukin 6
  • interleukin 6 (interferon, beta 2)
  • interleukin-6
  • Il6
  • IL6
  • LOC517016
  • il-6
Reaktivität
Human
577
238
115
37
29
26
22
10
8
7
7
6
4
3
3
2
1
Wirt
Kaninchen
384
299
201
36
7
7
5
Klonalität
Polyklonal
Konjugat
Dieser Interleukin 6 Antikörper ist konjugiert mit HRP
106
40
26
22
15
13
13
11
11
9
9
9
7
7
6
5
5
5
5
5
4
4
4
3
3
3
3
3
3
2
2
2
2
Applikation
Neutralization (Neut), Immunohistochemistry (IHC), Radioimmunoassay (RIA), ELISA, Western Blotting (WB)
593
537
224
152
123
97
70
66
59
48
41
38
19
17
14
13
12
8
8
5
4
4
4
4
3
3
2
2
2
2
2
1
1
1
1
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-6 produced in E.coli.
Immunogen Type: RecombinantProtein
Isotyp IgG
Spezifität This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human Il-6 in cell supernatants and certain body fluids. In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-6 will appear due to the variable amount of glycosylation on the molecule.  A control of similarly diluted normal rabbit IgG is recommended. This product has minimal reactivity with mouse IL-6.
Produktmerkmale This purified antibody has been tested for use in ELISA and western blotting. Reactivity is also expected in neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be < 10 pg/µL by the LAL method. By western blot a band approximately 23.7 kDa in size corresponding to native human IL-6 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
Reinigung purified
Andere Bezeichnung IL-6 (IL6 Antibody Abstract)
Hintergrund IL-6 is a secreted cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response and plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. IL-6 induces myeloma and plasmacytoma growth and induces nerve cells differentiation and acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. IL-6 also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.
Synonyms: HSF, Hybridoma growth factor, Hybridoma plasmacytoma growth factor, IFNB2, IL 6, IL6 protein, Interferon beta 2, Interleukin 6, BSF2, CDF
Gen-ID 3569
NCBI Accession NP_000591
UniProt P05231
Forschungsgebiet Cytokines, Immunology, Virology, Inflammation, Cancer, Receptors
Pathways Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagie, Cell RedoxHomeostasis
Applikationshinweise This purified antibody has been tested for use in ELISA and western blotting. Reactivity is also expected in neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be < 10 pg/µL by the LAL method. By western blot a band approximately 23.7 kDa in size corresponding to native human IL-6 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 100 µL
Konzentration 1.1 mg/ml
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Konservierungsmittel Gentamicin sulfate
Handhabung Do NOT add Sodium Azide!
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
Haltbarkeit 12 months
Bilder des Herstellers
Western Blotting (WB) image for anti-Interleukin 6 (IL6) antibody (HRP) (ABIN1043799) Western Blot of Peroxidase conjugated Rabbit anti-Human IL-6 antibody. Lane 1: Human ...
Produkt verwendet in: Novick: "Current concepts in the management of renovascular hypertension and ischemic renal failure." in: American journal of kidney diseases : the official journal of the National Kidney Foundation, Vol. 13, Issue 6 Suppl 1, pp. 33-7, 1989 (PubMed).

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