anti-Human Interferon Regulatory Factor 3 Antikörper für Western Blotting

Recommended Interferon Regulatory Factor 3 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Interferon Regulatory Factor 3 (IRF3) Antikörper
  • irf3
  • IRF3
  • C920001K05Rik
  • IRF-3
  • interferon regulatory factor 3
  • interferon regulatory factor 7
  • irf3
  • IRF3
  • irf-3
  • Irf3
  • IRF7
Reaktivität
Human
298
127
76
6
6
6
3
2
2
1
1
1
1
Wirt
Kaninchen
267
61
3
Klonalität
Polyklonal
Konjugat
Unkonjugiert
7
6
6
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Applikation
Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
273
132
108
81
49
40
28
26
25
14
14
8
4
3
3
3
2
2
1
Optionen
Hersteller
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Bilder

Produktnummer ABIN5663780
$ 393.25
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
29.632376 ABIN6142528 ChIP IHC WB Rabbit Anmelden zum Anzeigen Polyclonal 1
23.632376 ABIN3023115 IHC WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 2
22.132376 ABIN930915 DB ICC FACS IP WB Mouse IgG1 Anmelden zum Anzeigen IRF35I218 0
21.250505 ABIN742688 IF (cc) IF (p) IHC (p) WB Rabbit IgG AA 375-425, pSer396 Anmelden zum Anzeigen Polyclonal 4
19.750505 ABIN1873267 IF IHC WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 0
19.750505 ABIN5998004 IF/ICC IHC WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 0
19.132376 ABIN3185227 ELISA IHC WB Rabbit IgG Ser605 Anmelden zum Anzeigen Polyclonal 0
19.132376 ABIN933729 IF IP ELISA WB Mouse IgG1 kappa AA 108-166 Anmelden zum Anzeigen 3F10 0
19 ABIN1498899 IF IHC IHC (p) WB Mouse IgG2a Anmelden zum Anzeigen 2G3 0
16.88187 ABIN3023613 ICC WB Rabbit IgG pSer386 Anmelden zum Anzeigen Polyclonal 2
16.88187 ABIN6136112 WB Rabbit pSer396 Anmelden zum Anzeigen Polyclonal 1
16.88187 ABIN6142529 IF IHC WB Rabbit Anmelden zum Anzeigen Polyclonal 2
16.750505 ABIN4899543 CyTOF FACS ICC WB Goat IgG AA 206-427 Anmelden zum Anzeigen Polyclonal 0
16.750505 ABIN517226 IF IP ELISA WB Mouse IgG2a kappa AA 1-452 Anmelden zum Anzeigen 3C8 0
16.132376 ABIN3182757 ELISA IF IHC WB Rabbit IgG pSer385 Anmelden zum Anzeigen Polyclonal 0
16.132376 ABIN3185228 ELISA IHC WB Rabbit IgG Ser608 Anmelden zum Anzeigen Polyclonal 0
14.5 ABIN682588 IF (p) IHC (p) WB Rabbit IgG AA 60-95 Anmelden zum Anzeigen Polyclonal 1
13.750505 ABIN967285 Inhibition IF IP WB Mouse IgG1 AA 56-427 Anmelden zum Anzeigen SL-12-1 8
13.750505 ABIN6255286 ELISA ICC IF IHC WB Rabbit IgG pSer385 Anmelden zum Anzeigen Polyclonal 0
13.750505 ABIN6262651 ELISA ICC IF IHC WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 0

General

Antigen Interferon Regulatory Factor 3 (IRF3) Antikörper
Reaktivität Human
(298), (127), (76), (6), (6), (6), (3), (2), (2), (1), (1), (1), (1)
Wirt Kaninchen
(267), (61), (3)
Klonalität
Polyklonal
Konjugat Unkonjugiert
(7), (6), (6), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Applikation Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(273), (132), (108), (81), (49), (40), (28), (26), (25), (14), (14), (8), (4), (3), (3), (3), (2), (2), (1)
Pubmed 2 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails

Antigendetails Anwendungsinformationen Handhabung Referenzen Bilder
Reinigung Affinity purification
Immunogen Fusion protein of human IRF-3
Isotyp IgG

Antigendetails

Produktdetails Anwendungsinformationen Handhabung Referenzen Bilder zurück nach oben
Antigen
Andere Bezeichnung IRF-3 (IRF3 Antibody Abstract)
Hintergrund Interferon regulatory factors (IRFs) comprise a family of transcription factors that function within the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection. IRFs play an important role in pathogen defense, autoimmunity, lymphocyte development, cell growth, and susceptibility to transformation. The IRF family includes nine members: IRF-1, IRF-2, ISGF3gamma/p48, IRF-3, IRF-4 (Pip/LSIRF/ICSAT), IRF-5, IRF-6, IRF-7, and IRF-8/ICSBP. All IRF proteins share homology in their amino-terminal DNA-binding domains. IRF family members regulate transcription through interactions with proteins that share similar DNA-binding motifs, such as IFN-stimulated response elements (ISRE), IFN consensus sequences (ICS), and IFN regulatory elements (IRF-E). IRF-3 can inhibit cell growth and plays a critical role in controlling the expression of genes in the innate immune response. In unstimulated cells, IRF-3 is present in the cytoplasm. Viral infection results in phosphorylation of IRF-3 and leads to its translocation to the nucleus where it activates promoters containing IRF-3-binding sites. Phosphorylation of IRF-3 occurs at a cluster of carboxyl-terminal serine and threonine residues (between 385 and 405) leading to its association with the p300/CBP coactivator protein that promotes DNA binding and transcriptional activity. During infection, IRF-3 is likely activated through a pathway that includes activation of Toll-like receptors and of a kinase complex that includes IKKepsilon and TBK1. IRF-3 is phosphorylated at Ser396 following viral infection, expression of viral nucleocapsid, and double stranded RNA treatment. These events likely play a role in activation of IRF-3.
Synonyms: IRF-3 , IRF3, interferon regulatory factor 3
Gen-ID 3661
UniProt Q14653
Pathways TLR Signalweg, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades

Anwendungsinformationen

Produktdetails Antigendetails Handhabung Referenzen Bilder zurück nach oben
Applikations-hinweise Recommended dilutions: WB: 1:500-1:1000, IHC: 1:50-1:100, IF: 1:20-1:50
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails Antigendetails Anwendungsinformationen Referenzen Bilder zurück nach oben
Format Liquid
Buffer PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.3
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid freeze / thaw cycles
Lagerung -20 °C/-80 °C

Referenzen

Produktdetails Antigendetails Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Zhu, Wang, Pei, Wang, Wu, Qiu, Zhang, Lv, Li, Zhang: "Neddylation controls basal MKK7 kinase activity in breast cancer cells." in: Oncogene, Vol. 35, Issue 20, pp. 2624-33, 2017 Von den Autoren verwendete Methode: Western Blotting (WB)

Zhang, Wang, Zhong, Luo, Shang, Liu, Chen, Fang, Xiao: "Ubiquitin-specific Protease 15 Negatively Regulates Virus-induced Type I Interferon Signaling via Catalytically-dependent and -independent Mechanisms." in: Scientific reports, Vol. 5, pp. 11220, 2016 Von den Autoren verwendete Methode: Western Blotting (WB)

Bilder

Produktdetails Antigendetails Anwendungsinformationen Handhabung Referenzen zurück nach oben
Bilder des Herstellers
Western Blotting (WB) image for anti-Interferon Regulatory Factor 3 (IRF3) antibody (ABIN5663780) anti-Interferon Regulatory Factor 3 (IRF3) antibody
Western Blotting (WB) image for anti-Interferon Regulatory Factor 3 (IRF3) antibody (ABIN5663780) Western blot analysis of extracts of various cell lines, using IRF3 antibody.
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Interferon Regulatory Factor 3 (IRF3) antibody (ABIN5663780) Immunohistochemistry of paraffin-embedded human breast using IRF3 antibody.
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Interferon Regulatory Factor 3 (IRF3) antibody (ABIN5663780) Immunohistochemistry of paraffin-embedded human stomach using IRF3 antibody.
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Interferon Regulatory Factor 3 (IRF3) antibody (ABIN5663780) Immunohistochemistry of paraffin-embedded human gastric cancer using IRF3 antibody.
Immunofluorescence (IF) image for anti-Interferon Regulatory Factor 3 (IRF3) antibody (ABIN5663780) Immunofluorescence analysis of HeLa cells using IRF3 antibody.