anti-Human Interferon Regulatory Factor 3 Antikörper für Western Blotting

Recommended Interferon Regulatory Factor 3 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Interferon Regulatory Factor 3 (IRF3) Antikörper
  • irf3
  • IRF3
  • C920001K05Rik
  • IRF-3
  • interferon regulatory factor 3
  • interferon regulatory factor 7
  • irf3
  • IRF3
  • irf-3
  • Irf3
  • IRF7
Epitop
AA 56-427
26
25
21
20
19
18
17
15
15
11
9
5
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human
310
121
69
6
6
6
3
2
2
1
1
1
1
Wirt
Maus
255
85
3
Klonalität
Monoklonal
Konjugat
Unkonjugiert
7
6
6
5
4
4
4
4
4
4
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Applikation
Blocking Antibody (Inhibition), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
280
134
98
74
51
44
41
31
27
14
14
8
4
3
3
2
2
1
Optionen
Hersteller
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Bilder

Produktnummer ABIN967285
Preis und Verfügbarkeit auf Anfrage.
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
3.1680813 ABIN2779404 WB Rabbit C-Term Anmelden zum Anzeigen Polyclonal 1
3.1680813 ABIN742688 IF (cc) IF (p) IHC (p) WB Rabbit IgG AA 375-425, pSer396 Anmelden zum Anzeigen Polyclonal 4
3.1680813 ABIN3222919 IHC WB Rabbit C-Term Anmelden zum Anzeigen Polyclonal 0
3.1680813 ABIN153210 ICC IF IHC IHC (p) WB Rabbit AA 100-150 Anmelden zum Anzeigen Polyclonal 0
3.1680813 ABIN1873267 IF IHC WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 0
3.1680813 ABIN1532452 IHC ELISA WB Rabbit IgG AA 362-411 Anmelden zum Anzeigen Polyclonal 0
3.1680813 ABIN1532350 IF IHC ELISA WB Rabbit IgG AA 351-400 Anmelden zum Anzeigen Polyclonal 0
3.1680813 ABIN317099 EIA WB Mouse IgG1 AA 108-166 Anmelden zum Anzeigen 3F10 2
3.1680813 ABIN1531435 ELISA WB Rabbit IgG AA 362-411, pSer396 Anmelden zum Anzeigen Polyclonal 0
3.1680813 ABIN1533122 IHC ELISA WB Rabbit IgG AA 352-401 Anmelden zum Anzeigen Polyclonal 0
3.1680813 ABIN1531329 IF IHC ELISA WB Rabbit IgG AA 351-400, pSer385 Anmelden zum Anzeigen Polyclonal 0
3.1680813 ABIN1532087 ELISA WB Rabbit IgG AA 352-401, pSer386 Anmelden zum Anzeigen Polyclonal 0
3.1680813 ABIN316093 ELISA ICC IF IP WB Mouse IgG1 kappa AA 108-166 Anmelden zum Anzeigen 3F10 0
3.1680813 ABIN1868749 ICC IHC WB Rabbit IgG AA 1-360 Anmelden zum Anzeigen Polyclonal 0
3.1680813 ABIN3043039 WB Rabbit IgG AA 409-427, C-Term Anmelden zum Anzeigen Polyclonal 0
3.1680813 ABIN948044 IF WB Mouse AA 1-427, full length Anmelden zum Anzeigen Polyclonal 0
3.1680813 ABIN2668684 WB Rabbit AA 306-312 Anmelden zum Anzeigen Polyclonal 1
3.1680813 ABIN4899542 CyTOF FACS WB Mouse IgG2b AA 206-427 Anmelden zum Anzeigen 482205 0
3.1680813 ABIN4899543 CyTOF FACS ICC WB Goat IgG AA 206-427 Anmelden zum Anzeigen Polyclonal 0
3.1680813 ABIN517226 IF IP ELISA WB Mouse IgG2a kappa AA 1-452, full length Anmelden zum Anzeigen 3C8 0

General

Antigen Interferon Regulatory Factor 3 (IRF3) Antikörper
Epitop AA 56-427
(26), (25), (21), (20), (19), (18), (17), (15), (15), (11), (9), (5), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Human
(310), (121), (69), (6), (6), (6), (3), (2), (2), (1), (1), (1), (1)
Wirt Maus
(255), (85), (3)
Klonalität (Klon)
Monoklonal   ( )
Konjugat Unkonjugiert
(7), (6), (6), (5), (4), (4), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation Blocking Antibody (Inhibition), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(280), (134), (98), (74), (51), (44), (41), (31), (27), (14), (14), (8), (4), (3), (3), (2), (2), (1)
Pubmed 8 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails

Antigendetails Anwendungsinformationen Handhabung Referenzen Bilder
Produktmerkmale 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Reinigung Purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human IRF-3 recombinant fusion protein
Klon SL-12-1
Isotyp IgG1

Antigendetails

Produktdetails Anwendungsinformationen Handhabung Referenzen Bilder zurück nach oben
Antigen
Andere Bezeichnung IRF-3 (IRF3 Antibody Abstract)
Hintergrund Viral infection in mammals can lead to the induction of multiple pathways as part of the host defense mechanism. One of the major pathways activated is the JAK-STAT pathway by various interferons (IFNalpha and IFNbeta). These IFNs exert their influence via transcriptional activation of specific target genes involved in antiviral defense, for example the chemokine ISG15 gene or the major histocompatibility complex class I and II molecules. These genes in turn are regulated by the JAK-STAT signaling pathway and through interferon regulatory factors (IRFs). IRFs are a family of transcription factors that possess a broad range of activities. IRF-3 is one of nine members which all share a common DNA binding domain which binds to an IFN stimulated response element (ISRE) found in the majority of IFN-inducible promoters. The IRF-3 gene expresses a 50 kDa protein which is constitutively expressed in all tissues. The protein undergoes post-translational modification as well as dimerization and is translocated from the cytoplasm to the nucleus upon viral infection or exposure to dsRNA. The antibody recognizes human IRF-3. A purified recombinant IRF-3 fusion protein corresponding to human IRF-3 (amino acids 56-427) was used as the immunogen.
Molekulargewicht 50 kDa
Pathways TLR Signalweg, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades

Anwendungsinformationen

Produktdetails Antigendetails Handhabung Referenzen Bilder zurück nach oben
Applikations-hinweise Clone SL-12.1 can be used for western blot analysis (0.5-2.0 µg/ml). Other reported applications include immunoprecipitation and immunofluorescence microscopy. The antibody will recognize both the nuclear and cytoplasmic forms of IRF-3 and can block IRF-3 DNA binding. Jurkat cells are recommended as a positive control (Cat, No. ABIN968537).
Kommentare

Related Products: ABIN968537, ABIN967389

Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails Antigendetails Anwendungsinformationen Referenzen Bilder zurück nach oben
Format Liquid
Konzentration 0.5 mg/ml
Buffer Aqueous buffered solution.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C

Referenzen

Produktdetails Antigendetails Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Hiscott, Pitha, Genin, Nguyen, Heylbroeck, Mamane, Algarte, Lin: "Triggering the interferon response: the role of IRF-3 transcription factor." in: Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research, Vol. 19, Issue 1, pp. 1-13, 1999

Mamane, Heylbroeck, Génin, Algarté, Servant, LePage, DeLuca, Kwon, Lin, Hiscott: "Interferon regulatory factors: the next generation." in: Gene, Vol. 237, Issue 1, pp. 1-14, 1999

Ronco, Karpova, Vidal, Howley: "Human papillomavirus 16 E6 oncoprotein binds to interferon regulatory factor-3 and inhibits its transcriptional activity." in: Genes & development, Vol. 12, Issue 13, pp. 2061-72, 1998

Wathelet, Lin, Parekh, Ronco, Howley, Maniatis: "Virus infection induces the assembly of coordinately activated transcription factors on the IFN-beta enhancer in vivo." in: Molecular cell, Vol. 1, Issue 4, pp. 507-18, 1998

Au, Moore, Lowther, Juang, Pitha: "Identification of a member of the interferon regulatory factor family that binds to the interferon-stimulated response element and activates expression of interferon-induced genes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 25, pp. 11657-61, 1996

Darnell, Kerr, Stark: "Jak-STAT pathways and transcriptional activation in response to IFNs and other extracellular signaling proteins." in: Science (New York, N.Y.), Vol. 264, Issue 5164, pp. 1415-21, 1994

Loh, Chang, Fodor, Flavell: "Dissection of the interferon gamma-MHC class II signal transduction pathway reveals that type I and type II interferon systems share common signalling component(s)." in: The EMBO journal, Vol. 11, Issue 4, pp. 1351-63, 1992

Reich, Evans, Levy, Fahey, Knight, Darnell: "Interferon-induced transcription of a gene encoding a 15-kDa protein depends on an upstream enhancer element." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, Issue 18, pp. 6394-8, 1987

Bilder

Produktdetails Antigendetails Anwendungsinformationen Handhabung Referenzen zurück nach oben
Bilder des Herstellers
Western Blotting (WB) image for anti-Interferon Regulatory Factor 3 (IRF3) (AA 56-427) antibody (ABIN967285) Western blot analysis of IRF-3. Lysates from Jurkat cells were probed with anti-human...