anti-Ratte (Rattus) Cytoplasmic Protein NCK1 Antikörper für Immunofluorescence

Recommended Cytoplasmic Protein NCK1 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
NCK Adaptor Protein 1 (NCK1) Antikörper
  • NCK
  • NCKalpha
  • nck-1
  • NCK1
  • 6330586M15Rik
  • D230010O13Rik
  • Nck
  • Nck-1
  • Nck1
  • nck
  • nck1-a
  • nck1-b
  • nckalpha
  • NCK adaptor protein 1
  • non-catalytic region of tyrosine kinase adaptor protein 1
  • NCK adaptor protein 1b
  • NCK adaptor protein 1 L homeolog
  • NCK1
  • Nck1
  • nck1b
  • nck1.L
Epitop
AA 279-377
24
21
10
9
7
5
4
3
2
2
2
1
1
1
1
1
1
Reaktivität
Huhn, Rind (Kuh), Hund, Frosch, Human, Maus, Ratte (Rattus)
150
34
27
19
7
4
4
3
3
2
2
2
2
2
1
1
1
1
Wirt
Maus
81
68
1
Klonalität
Monoklonal
Konjugat
Dieser Cytoplasmic Protein NCK1 Antikörper ist unkonjugiert
6
6
6
6
6
4
2
2
2
2
2
2
2
2
2
2
2
2
1
Applikation
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
144
94
69
28
14
14
9
6
5
2
2
1
1
Optionen
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Produktnummer ABIN967744
Preis und Verfügbarkeit auf Anfrage.
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
11.795858 ABIN967743 IF IP IHC WB Mouse IgG2b AA 279-377 Anmelden zum Anzeigen 108-NCK 5
11.795858 ABIN3197734 IC IF WB Rabbit Anmelden zum Anzeigen Polyclonal 0
1 ABIN1873839 IF IHC WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 0
1 ABIN2888058 IF IHC WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 0
1 ABIN5697483 ELISA FACS IF IHC IP WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 0
1 ABIN1089241 IF IHC ELISA WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 0

General

Antigen NCK Adaptor Protein 1 (NCK1) Antikörper
Epitop AA 279-377
(24), (21), (10), (9), (7), (5), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reaktivität Huhn, Rind (Kuh), Hund, Frosch, Human, Maus, Ratte (Rattus)
(150), (34), (27), (19), (7), (4), (4), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Wirt Maus
(81), (68), (1)
Klonalität (Klon)
Monoklonal   ( )
Konjugat Dieser Cytoplasmic Protein NCK1 Antikörper ist unkonjugiert
(6), (6), (6), (6), (6), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Applikation Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(144), (94), (69), (28), (14), (14), (9), (6), (5), (2), (2), (1), (1)
Pubmed 5 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-Cytoplasmic Protein NCK1 Antikörper

Target Details Cytoplasmic Protein NCK1 Anwendungsinformationen Handhabung Referenzen für anti-Cytoplasmic Protein NCK1 Antikörper (ABIN967744) Bilder
Kreuzreaktivität Rind (Kuh), Huhn, Hund, Frosch, Maus, Ratte (Rattus)
Produktmerkmale 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Reinigung The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human NCK aa. 279-377
Klon 108-NCK
Isotyp IgG2b

Target Details Cytoplasmic Protein NCK1

Produktdetails anti-Cytoplasmic Protein NCK1 Antikörper Anwendungsinformationen Handhabung Referenzen für anti-Cytoplasmic Protein NCK1 Antikörper (ABIN967744) Bilder zurück nach oben
Antigen
Andere Bezeichnung NCK (NCK1 Antibody Abstract)
Hintergrund NCK is a 47 kDa protein that belongs to a class of signaling molecules called adaptors. These proteins exert their effects by coupling activated receptors to downstream effectors. NCK is comprised almost entirely of one SH2 and three SH3 domains and it possesses no intrinsic catalytic activity. Investigations of its interactions with cellular proteins have found constitutive association of NCK with various Ser/THr kinases, such as p21-associated kinase (PAK) and casein kinase 1. Other proteins that directly interact with NCK are: Sos, Cbl, Wiskott-Aldrich syndrome protein (WASP), IRS1, and multiple tyrosine kinase receptors. The presence of NCK is necessary for the mitogenic response elicited by PDGF in some cells. In addition, the Drosophila homologue of NCK is involved in regulating cytoskeletal organization. This antibody is routinely tested by western blot analysis.
Molekulargewicht 47 kDa
Pathways T-Zell Rezeptor Signalweg, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals

Anwendungsinformationen

Produktdetails anti-Cytoplasmic Protein NCK1 Antikörper Target Details Cytoplasmic Protein NCK1 Handhabung Referenzen für anti-Cytoplasmic Protein NCK1 Antikörper (ABIN967744) Bilder zurück nach oben
Kommentare

Related Products: ABIN967389

Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-Cytoplasmic Protein NCK1 Antikörper Target Details Cytoplasmic Protein NCK1 Anwendungsinformationen Referenzen für anti-Cytoplasmic Protein NCK1 Antikörper (ABIN967744) Bilder zurück nach oben
Format Liquid
Konzentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store undiluted at -20° C.

Referenzen für anti-Cytoplasmic Protein NCK1 Antikörper (ABIN967744)

Produktdetails anti-Cytoplasmic Protein NCK1 Antikörper Target Details Cytoplasmic Protein NCK1 Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Dalva, Takasu, Lin, Shamah, Hu, Gale, Greenberg: "EphB receptors interact with NMDA receptors and regulate excitatory synapse formation." in: Cell, Vol. 103, Issue 6, pp. 945-56, 2001

Miyoshi-Akiyama, Aleman, Smith, Adler, Mayer: "Regulation of Cbl phosphorylation by the Abl tyrosine kinase and the Nck SH2/SH3 adaptor." in: Oncogene, Vol. 20, Issue 30, pp. 4058-69, 2001

Schmitz, Thömmes, Beier, Wagner, Sachinidis, Düsing, Vetter: "Angiotensin II-induced stimulation of p21-activated kinase and c-Jun NH2-terminal kinase is mediated by Rac1 and Nck." in: The Journal of biological chemistry, Vol. 276, Issue 25, pp. 22003-10, 2001

McManus, Boerner, Danielsen, Wang, Matsumura, Maihle: "An oncogenic epidermal growth factor receptor signals via a p21-activated kinase-caldesmon-myosin phosphotyrosine complex." in: The Journal of biological chemistry, Vol. 275, Issue 45, pp. 35328-34, 2000

Roche, McGlade, Jones, Gish, Pawson, Courtneidge et al.: "Requirement of phospholipase C gamma, the tyrosine phosphatase Syp and the adaptor proteins Shc and Nck for PDGF-induced DNA synthesis: evidence for the existence of Ras-dependent and Ras-independent ..." in: The EMBO journal, Vol. 15, Issue 18, pp. 4940-8, 1996

Bilder

Produktdetails anti-Cytoplasmic Protein NCK1 Antikörper Target Details Cytoplasmic Protein NCK1 Anwendungsinformationen Handhabung Referenzen für anti-Cytoplasmic Protein NCK1 Antikörper (ABIN967744) zurück nach oben
Bilder des Herstellers
Western Blotting (WB) image for anti-NCK Adaptor Protein 1 (NCK1) (AA 279-377) antibody (ABIN967744) Western blot anlaysis of NCK on HeLa lysate. Lane 1: 1:1000, lane 2: 1: 2000, lane 3:...
Immunofluorescence (IF) image for anti-NCK Adaptor Protein 1 (NCK1) (AA 279-377) antibody (ABIN967744) Immunofluorescent staining on HISM cells.
 image for anti-NCK Adaptor Protein 1 (NCK1) (AA 279-377) antibody (ABIN967744) anti-NCK Adaptor Protein 1 (NCK1) (AA 279-377) antibody (Image 3)
Western Blotting (WB) image for anti-NCK Adaptor Protein 1 (NCK1) (AA 279-377) antibody (ABIN967744) anti-NCK Adaptor Protein 1 (NCK1) (AA 279-377) antibody (Image 4)