Esel anti-Schaf IgG (Heavy & Light Chain) Antikörper (Texas Red (TR)) - Preadsorbed
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- Target Alle IgG Produkte
- IgG
- Fragment
- F(ab')2 fragment
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Bindungsspezifität
- Heavy & Light Chain
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Reaktivität
- Schaf
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Wirt
- Esel
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Klonalität
- Polyklonal
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Konjugat
- Texas Red (TR)
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Applikation
- Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
- Spezifität
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Sheep IgG and Sheep Serum.
- Produktmerkmale
- This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
- Aufreinigung
- Preadsorption: Solid phase absorption
- Immunogen
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Immunogen: Sheep IgG whole molecule
- Isotyp
- IgG
- Labeling Ratio
- 3.1
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- Applikationshinweise
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Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
FLISA Dilution: 1:10,000 - 1:50,000
Flow Cytometry Dilution: 1:500 - 1:2,500
IF Microscopy Dilution: 1:1,000 - 1:5,000
- Kommentare
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Texas Red™ is a registered trademark of Molecular Probes Inc.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
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Reconstitution Volume: 500 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Konzentration
- 1.0 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
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Avoid cycles of freezing and thawing.
This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
Product is photosensitive and should be protected from light. - Lagerung
- RT,4 °C,-20 °C
- Informationen zur Lagerung
- Store the vial at -20 °C or below after dilution.
- Haltbarkeit
- 12 months
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- Target
- IgG
- Abstract
- IgG Produkte
- Substanzklasse
- Antibody
- Hintergrund
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Synonyms: Donkey F(ab')2 Anti-Sheep IgG Texas Red™ Conjugated Antibody, Donkey Fab2 Anti-Sheep IgG Antibody Texas Red™ Conjugation
Background: F(ab')2 Anti-Sheep IgG (H&L) Antibody generated in donkey detects sheep IgG. Representing approximately 75 % of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunofluorescence, IHC, and other immunoassays. This F(ab')2 Anti-Sheep IgG Antibody is conjugated to Texas Red™.
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