Kaninchen anti-Pferd IgG Antikörper (Cy3) - Preadsorbed

Produktnummer ABIN6698832

Produktdetails

Target: IgG

Fragment: F(ab')2 fragment

Reaktivität: Pferd

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Cy3

Applikation: Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)

Produktmerkmale:

Synonyms: Rabbit F(ab')2 Anti-Horse IgG Antibody CY3 Conjugation, Rabbit F(ab')2 Anti-Horse IgG CY3 Conjugated Antibody, Rabbit Fab2 Anti-Horse IgG CY3 Conjugated Antibody

Background: F(ab')2 Anti-Horse IgG Cy3 Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.

Aufreinigung: Preadsorption: Solid phase absorption

Immunogen:

Immunogen: Horse IgG whole molecule

Isotyp: IgG

Labeling Ratio: 5.1

Anwendungsinformationen

Applikationshinweise:

Application Note: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.

FLISA Dilution: 1:10,000 - 1:50,000

Flow Cytometry Dilution: 1:500 - 1:2,500

IF Microscopy Dilution: 1:1,000 - 1:5,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 250 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Antigendetails

Target: IgG

Abstract: IgG Produkte

Substanzklasse: Antibody