Huhn anti-Kaninchen IgG (Heavy & Light Chain) Antikörper (HRP)

Produktnummer ABIN619641

Produktdetails

Target: IgG

Bindungsspezifität: Heavy & Light Chain

Reaktivität: Kaninchen

Wirt: Huhn

Klonalität: Polyklonal

Konjugat: HRP

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Kreuzreaktivität: Human, Maus

Homologie: rabbit IgG (H&L)

Produktmerkmale: Chicken anti-rabbit IgG (H&L) is a secondary antibody conjugated to HRP which binds to rabbit IgG (H&L) in immunological assays.

Aufreinigung: Affinity purification

Immunogen: Purified Rabbit IgG

Isotyp: IgY

Anwendungsinformationen

Applikationshinweise: optimal working dilution should be determined by the investigator

Kommentare:

This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin rabbit serum proteins and human and mouse serum and IgG on immunoelectrophoresis.This antibody was absorbed against solid phase human and mouse serum and IgG.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution please add 0.55 mL of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.

Handhabung: Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
A solution with 50 % glycerol will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol.

Lagerung: 4 °C

Informationen zur Lagerung: Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0.5 mg of antibody in 0.55 ml of sterile water add 0.55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.

Antigendetails

Target: IgG

Abstract: IgG Produkte

Substanzklasse: Antibody