Maus anti-Human IgM Antikörper
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- Target Alle IgM Produkte
- IgM
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Reaktivität
- Human
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Wirt
- Maus
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Klonalität
- Monoklonal
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Applikation
- Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Verwendungszweck
- Mouse Monoclonal anti-Human IgM Immunoglobulin (IM260), Purified, BSA-free
- Produktmerkmale
- This antibody recognizes a protein of 75 kDa, identified as mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This MAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Aufreinigung
- Purified
- Immunogen
- Recombinant heavy chain of human IgM
- Klon
- IM260
- Isotyp
- IgG1 kappa
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- Applikationshinweise
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Immunohistology formalin-fixed 0.5-1 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Western blotting 0.5-1 μg/mL
- Optimal dilution for a specific application should be determined by user
- Kommentare
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293T, Raji or hPBL cells. Tonsil or Spleen
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 1 mg/mL
- Buffer
- PBS (no BSA, no azide)
- Konservierungsmittel
- Azide free
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- Target
- IgM
- Andere Bezeichnung
- IgM Immunoglobulin (IgM Produkte)
- Substanzklasse
- Antibody
- Hintergrund
- AGM1, IGHM, Constant Region of Heavy Chain of IgM, Ig Mu Chain C Region
- Molekulargewicht
- 50-75 kDa
- Gen-ID
- 3507, 510635
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