Immunofluorescence (IF), Western Blotting (WB), Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Immunodiffusion (ID), Dot Blot (DB)
Aufreinigung
Hyperimmune antisera with strong precipitating activity are selected for Fractionation by Salt-Precipitation and DEAE-Chromatography
Immunogen
A pool of purified Bence Jones kappa proteins isolated from human urine. Freund’s complete adjuvant is used in the first step of the immunization procedure.