Ziege anti-Human IgA, IgG, IgM (Heavy & Light Chain) Antikörper (Alkaline Phosphatase (AP)) - Preadsorbed
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- Target Alle IgA, IgG, IgM Produkte
- IgA, IgG, IgM
- Fragment
- F(ab')2 fragment
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Bindungsspezifität
- Heavy & Light Chain
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Reaktivität
- Human
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Wirt
- Ziege
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Klonalität
- Polyklonal
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Konjugat
- Alkaline Phosphatase (AP)
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Applikation
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- Produktmerkmale
- Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
- Aufreinigung
- Preadsorption: Solid phase absorption
- Immunogen
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Immunogen: Human IgG, IgA, and IgM whole molecules
- Isotyp
- IgG
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- Applikationshinweise
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Immunohistochemistry Dilution: 1:200 - 1:1,000
Application Note: F(ab')2 Anti-Human IgG IgA IgM (H&L) Alkaline Phosphate Antibody has been assayed against 1.0 μg of Human IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product. Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
ELISA Dilution: 1:2,000 - 1:10,000
Western Blot Dilution: 1:500 - 1:2,500
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 0.5 mg/mL
- Buffer
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Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C
- Informationen zur Lagerung
- Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
- Haltbarkeit
- 12 months
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- Target
- IgA, IgG, IgM
- Andere Bezeichnung
- IgG IgA IgM (IgA, IgG, IgM Produkte)
- Substanzklasse
- Antibody
- Hintergrund
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Synonyms: Goat F(ab')2 Anti-Human IgG IgA IgM Alkaline phosphatase Conjugated Antibody Pre-Adsorbed, Goat Fab2 Anti-Human IgGAM Antibody alk phos Conjugation
Background: F(ab')2 Anti-Human IgG IgA IgM (H&L) Alkaline Phosphatase Antibody generated in goat detects human (heavy and light chain) immunoglobulin G, A, and M. Immunoglobulin G binds to antigens and can neutralize or opsinize targets, and are predominantly involved in the secondary immune response. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Immunoglobulin M, or IgM, is a pentamer composed of 5 immunoglobulin molecules linked through their F(c) domain by the J chain. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
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