Ziege anti-Kaninchen IgA, IgG, IgM (Fab Region), (Fc Region) Antikörper (Biotin) - Preadsorbed

Produktnummer ABIN458610

Produktdetails

Target: IgA, IgG, IgM

Bindungsspezifität: Fab Region, Fc Region

Reaktivität: Kaninchen

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Biotin

Applikation: ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Spezifität: The reactivity of the antiserum is directed to the Fc and Fab subunits of the major immunoglobulin isotypes.

Produktmerkmale: Biotin-conjugated IgG fraction of polyclonal goat antiserum to rabbit immunoglobulins, heavy and light chains
Biotin/IgG protein molar ratio (B/P) is approximately 6.4.

Aufreinigung: Preadsorption: Immunoaffinity adsorbed using insolubilized antigens.

Immunogen: Purified normal IgG, IgA and IgM isolated from pooled rabbit serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: In immunocytochemical and immunohistochemical staining to identify and measure immunoglobulins, antigen or antibody, at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases, to identify a specific antigen or immune complex using a reference antibody of rabbit origin in the middle layer of the indirect test procedure. As a second step an avidin or streptavidin conjugate of the user's choice has to be used. This immunoconjugate is not pre-diluted.
The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions fro histochemical and cytochemical use are usually between 1:100 and 1:500, in ELISA and comparable non-precipitating antibody-binding assays between 1:5,000 and 1:20,000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -24 °C.

Konzentration: 10 mg/mL

Buffer: Biotin-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).

Konservierungsmittel: Without preservative

Handhabung: Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The lyophilized conjugate is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.

Antigendetails

Target: IgA, IgG, IgM

Andere Bezeichnung: IgG + IgA + IgM (IgA, IgG, IgM Produkte)

Substanzklasse: Antibody