Kaninchen anti-Human IgG (Fc Region) Antikörper (Alkaline Phosphatase (AP))

Produktnummer ABIN339045

Produktdetails

Target: IgG

Bindungsspezifität: Fc Region

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Alkaline Phosphatase (AP)

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Spezifität: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-alkaline phosphatase (calf intestine), anti-rabbit serum, human IgG, human IgG F(c) and human serum. No reaction was observed against human IgG F (ab')2.

Aufreinigung: Immunoaffinity purified

Immunogen: Human IgG F(c) fragment.

Type of Immunogen: Purified protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (1:1600), IHC, WB

Usage: Suitable for use in Western Blot (Western or Dot Blot), ELISA and Immunohistochemistry. ELISA: 1:1600 to 1:16000 using pNPP, p-nitrophenyl phosphate, as a substrate for 30 minutes at room temperature. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: TBS, 1 mM magnesium chloride, 0.1 mM zinc chloride,  pH 8.0, 10 mg/mL BSA (IgG, protease free), 0.01 % sodium azide, 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 4°C. Do not freeze.

Antigendetails

Target: IgG

Abstract: IgG Produkte

Substanzklasse: Antibody