It has been selected for its ability to recognize IgG3 in immunohistochemical staining and Western blotting.
Aufreinigung
Affinity Chromatography
Immunogen
Native Protein IgG3 from Human Serum There are four Immunoglobulin G subclasses, each numbered for their relative abundance in the body. These molecules are an important part of the immune system. All Immunoglobulin G molecules are made up of four chains of amino acids, connected at a central hinge in such a way that three ends extend from the center. The hinge within the Immunoglobulin G3 antibody is by far the longest in all the Immunoglobulin G subclasses. The length of the hinge allows Immunoglobulin G3 to quickly and effectively bind to and eliminate antigens. On the other hand, the length of the hinge also makes this subclass of Immunoglobulin G the most susceptible to damage from other molecules. The antibody is a mouse monoclonal antibody raised against IgG3.
ELISA, WB, IF
Monoclonal
NI 330 (HP 6050)
IgG1 kappa
unconjugated
Applikationshinweise
Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200 Optimal working dilutions must be determined by end user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
500 µg/mL
Buffer
Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handhabung
Avoid repeated freeze/thaw cycles
Lagerung
4 °C
Informationen zur Lagerung
Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
There are four immunoglobulin G subclasses, each numbered for their relative abundance in the body. These molecules are an important part of the immune system. All immunoglobulin G molecules are made up of four chains of amino acids, connected at a central hinge in such a way that three ends extend from the center. The hinge within the immunoglobulin G3 antibody is by far the longest in all the immunoglobulin G subclasses. The length of the hinge allows immunoglobulin G3 to quickly and effectively bind to and eliminate antigens. On the other hand, the length of the hinge also makes this subclass of immunoglobulin G the most susceptible to damage from other molecules.