Ziege anti-Ratte IgA Antikörper (HRP)

Produktnummer ABIN300957

Produktdetails

Target: IgA

Reaktivität: Ratte

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: HRP

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Spezifität: Anti-Peroxidase, anti-Goat Serum, Rat IgA and Rat Serum. Specificity was confirmed by ELISA at less than 1 % cross reactivity against other Rat heavy or light chain isotypes

Aufreinigung: Immunoaffinity purified

Immunogen: Rat IgA alpha chain.

Type of Immunogen: Purified protein

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (1:1000 - 1:5000), IHC, WB

Usage: Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. This product has been assayed against 1.0 μg of Rat IgA in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Kommentare:

Target Species of Antibody: Rat

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: deionized water

Konzentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 10 mg/mL BSA (IgG, protease free), 0.01 % Thimerosal.

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze/thaw cycles. Store undiluted.

Antigendetails

Target: IgA

Abstract: IgA Produkte

Substanzklasse: Antibody