The RG7/7.6 antibody reacts strongly with rat Igs bearing - light chain of the Igk-1[a]allotype (e.g., AO, AUG, BN, BUF, LEW, LOU, PVG, WAG, WF), weakly with Igk-1[b] light chains (e.g., ACI, COP, DA, F344, SHR), and it does not react with λ light chain nor heavy chains. At the time that this antibody was characterized, the Igk-1[a] allotype was designated - 1b, and Igk-1[b] was -1a, the current nomenclature was standardized at the Fourth International Workshop on Allogenic Systems in the Rat. The RG7/7.6 antibody also strongly cross-reacts with Syrian and Armenian hamster Ig - light chain.
BD Pharmingen™ Biotin Mouse Anti-Rat and Anti-Hamster Ig, κ Light Chain - Biotin - Clone RG7/7.6 - Isotype Mouse IgG2a, κ - Reactivity Rat, Ar Ham, Syr Ham - 1 mL
Aufreinigung
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen
Pooled Rat IgG
Klon
RG7-7-6
Isotyp
IgG2a kappa
Applikationshinweise
Optimal working dilution should be determined by the investigator.
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
Lagerung
4 °C
Informationen zur Lagerung
Store undiluted at 4°C.
Butcher: "A list of monoclonal antibodies specific for alloantigens of the rat." in: Journal of immunogenetics, Vol. 14, Issue 2-3, pp. 163-76, (1988) (PubMed).
Springer, Bhattacharya, Cardoza, Sanchez-Madrid: "Monoclonal antibodies specific for rat IgG1, IgG2a, and IgG2b subclasses, and kappa chain monotypic and allotypic determinants: reagents for use with rat monoclonal antibodies." in: Hybridoma, Vol. 1, Issue 3, pp. 257-73, (1984) (PubMed).