Ziege anti-Ratte IgM (Fc Region) Antikörper (HRP)

Produktnummer ABIN2623712

Produktdetails

Target: IgM

Bindungsspezifität: Fc Region

Reaktivität: Ratte

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: HRP

Applikation: ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunodiffusion (ID), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Dot Blot (DB)

Spezifität: Rat IgM, Fc specific.

Aufreinigung: Purified IgG

Immunogen: Purified homogenous IgM isolated from pooled rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Type of Immunogen: Purified protein

Anwendungsinformationen

Applikationshinweise: Approved: DB, ELISA, ICC, ID, IHC, IHC-P

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. The reactivity of the antiserum is directed to the Fc subunit of the IgM molecule which expresses strict isotypic (class) specificity. It does not react with purified IgG, IgG/Fab fragments and IgA or any non-Ig protein in rat serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates. to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases. to identify a specific antigen using a reference antibody of rat origin known to be of the IgM isotype in the middle layer of the indirect test procedure. in non-isotopic assay methodology (e. g. ELISA) to measure IgM in rat serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labeled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:50 and 1:250. in ELISA and comparable non-precipitating antibody-binding assays between 1:1000 and 1:8000 depending on the method used.

Kommentare:

Target Species of Antibody: Rat

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2. No preservative added.

Konservierungsmittel: Without preservative

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Antigendetails

Target: IgM

Andere Bezeichnung: IgM, (IgM Produkte)

Substanzklasse: Antibody