anti-rat Ig chain monoclonal antibody recognizes a determinant on rat kappa chains and binds preferentially to the Ig-1b allotype. This antibody provides a suitable marker for virtually all immunoglobin, regardless of class since 5 % or less of rat light chains are lambda. ABIN133281 detects surface immunoglobulin of rat peripheral blood B lymphocytes and has been used in indirect binding assays for detecting rat antibodies. Applications include flow cytometry for directly labelling rat B lymphocytes and as an excellent specific secondary antibody for use with rat monoclonals.
Aufreinigung
Purified from ascitic fluid via Protein G Chromatography
Immunogen
Pooled rat serum IgG . Fusion Partner: P3-NSI-1-Ag4 (NS1/1) . Donor: BALB/c spleen
Optimal working dilutions should be determined experimentally by the investigator.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
0.1 mg/mL
Buffer
PBS, 0.02 % NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handhabung
Aliquot to Avoid repeated freezing and thawing.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store at 4°C. For long term storage aliquot and freeze unused portion at -20C in volumes appropriate for single usage. Avoid freeze/thaw cycles.