Suitable for Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase electronmicroscopy and Immunohistochemistry as well as other peroxidase-antibody basedenzymatic assays requiring lot-to-lot consistency. Recommended dilutionsELISA 1/10,000-1/70,000. Western blot 1/1,000-1/5,000. Immunohistochemistry 1/500-1/2,500.
Other applications not tested. Otimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Rekonstitution
Restore with 1.0 mL of deionized water (or equivalent).
Konzentration
2.0 mg/mL
Buffer
0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 containing 10 mg/mL BSA (IgG and Protease free) as stabilizer and 0.01 % (w/v) Gentamicin Sulfate as a preservative.
Konservierungsmittel
Gentamicin sulfate
Vorsichtsmaßnahmen
This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handhabung
Avoid repeated freezing and thawing. Dilute only prior to immediate use Centrifuge product if not completely clear after standing at room temperature. Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50 % and then aliquot contents and freeze at -28 °C or below. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
Smart, Goecke, Ramm, Petersen, Lenz, Haverich, Niemann, Hilfiker: "Dot blots of solubilized extracellular matrix allow quantification of human antibodies bound to epitopes present in decellularized porcine pulmonary heart valves." in: Xenotransplantation, Vol. 28, Issue 1, pp. e12646, (2021) (PubMed).
Ramm, Hartmann, Tudorache, Haverich, Hilfiker: "No evidence for αGal epitope transfer from media containing FCS onto human endothelial cells in culture." in: Xenotransplantation, Vol. 22, Issue 5, pp. 345-55, (2015) (PubMed).
von Rhein, Hunfeld, Ludwig: "Serologic evidence for effective production of cytolysin A in Salmonella enterica serovars typhi and paratyphi A during human infection." in: Infection and immunity, Vol. 74, Issue 11, pp. 6505-8, (2006) (PubMed).