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Ziege anti-Human IgG (Whole Molecule) Antikörper (Biotin) - Preadsorbed

ELISA, IHC, WB, DB, IM Polyclonal Biotin
Produktnummer ABIN117254
  • Target Alle IgG Produkte
    IgG
    Bindungsspezifität
    • 2272
    • 457
    • 363
    • 188
    • 130
    • 123
    • 93
    • 29
    • 28
    • 28
    • 17
    • 9
    • 6
    • 6
    • 6
    • 6
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    Whole Molecule
    Reaktivität
    • 673
    • 659
    • 556
    • 430
    • 366
    • 219
    • 199
    • 144
    • 134
    • 122
    • 109
    • 93
    • 93
    • 91
    • 84
    • 58
    • 45
    • 33
    • 27
    • 16
    • 12
    • 10
    • 8
    • 5
    • 5
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Wirt
    • 1661
    • 1418
    • 532
    • 217
    • 104
    • 90
    • 33
    • 30
    • 21
    • 16
    • 15
    • 11
    • 11
    • 1
    Ziege
    Klonalität
    • 3308
    • 194
    • 1
    Polyklonal
    Konjugat
    • 576
    • 401
    • 394
    • 351
    • 276
    • 245
    • 191
    • 119
    • 98
    • 66
    • 64
    • 61
    • 58
    • 52
    • 48
    • 47
    • 45
    • 44
    • 44
    • 41
    • 39
    • 34
    • 33
    • 33
    • 31
    • 30
    • 29
    • 29
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 27
    • 26
    • 26
    • 25
    • 21
    • 19
    • 17
    • 17
    • 16
    • 16
    • 15
    • 15
    • 14
    • 10
    • 9
    Biotin
    Applikation
    • 1778
    • 1713
    • 1690
    • 1444
    • 934
    • 910
    • 883
    • 463
    • 234
    • 115
    • 115
    • 100
    • 92
    • 86
    • 77
    • 50
    • 50
    • 32
    • 31
    • 27
    • 25
    • 21
    • 17
    • 16
    • 11
    • 8
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB), Immunomicroscopy (IM)
    Produktmerkmale
    Molar Ratio: 10-20 BAC molecules per Goat IgG molecule.
    Aufreinigung
    Preadsorption: Solid phase absorption
    Immunogen
    Human IgG, whole molecule.
  • Applikationshinweise
    Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well asother antibody based assays using streptavidin or avidin conjugates requiring lot-to-lotconsistency. Recommended Dilutions: This product has been assayed against 1.0 μg of Human IgG in astandard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6- sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1:20,000 to 1:80,000 of the reconstitutionconcentration is suggested for this product.

    Other applications not tested.
    Otimal dilutions are dependent on conditions and should be determined by the user.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Rekonstitution
    Restore with 1.0 mL of deionized water (or equivalent).
    Konzentration
    1.0 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) sodium azide, 10 mg/mL BSA (IgG and Protease free)
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    Handhabung
    Avoid repeated freezing and thawing.
    Lagerung
    4 °C/-20 °C
    Informationen zur Lagerung
    Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer.
  • Target
    IgG
    Abstract
    IgG Produkte
    Substanzklasse
    Antibody
    Hintergrund
    Synonyms: Human Immunoglobulin G
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