Kaninchen anti-Rind (Kuh) IgG (Fc Region) Antikörper (Biotin) - Preadsorbed

Produktnummer ABIN117044

Produktdetails

Target: IgG

Bindungsspezifität: Fc Region

Reaktivität: Rind (Kuh)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Biotin

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Produktmerkmale: Molar Ratio: 10-20 BAC molecules per Rabbit IgG molecule.

Aufreinigung: Preadsorption: Solid phase absorption

Immunogen: Bovine IgG F(c) fragment.

Anwendungsinformationen

Applikationshinweise: Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well asother antibody based assays using streptavidin or avidin conjugates requiring lot-to-lotconsistency. Note: This product has been assayed against 1.0 μg of Bovine IgG in a standard captureELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1/2,000 to 1/15,000 of the reconstitutionconcentration is suggested for this product. Recommended Dilutions: ELISA: 1/20,000-1/100,000
Western Blot: 1/2,000-1/10,000Immunohistochemistry: 1/1,000-1/5,000

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 1.0 mL of deionized water (or equivalent).

Konzentration: 1.5 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/mL Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01 % (w/v) sodium azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handhabung: Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.

Lagerung: 4 °C

Informationen zur Lagerung: Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50 % and then aliquot contents and freeze at -28 °C or below. This antibody is stable for one month at 2-8 °C as an undiluted liquid.

Antigendetails

Target: IgG

Abstract: IgG Produkte

Substanzklasse: Antibody

Hintergrund: Synonyms: Bovine Immunoglobulin G