Ziege anti-Maus IgG2b (Heavy Chain) Antikörper (Alkaline Phosphatase (AP)) - Preadsorbed

Produktnummer ABIN1118493

Produktdetails

Target: IgG2b

Bindungsspezifität: Heavy Chain

Reaktivität: Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Alkaline Phosphatase (AP)

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Spezifität: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum and Mouse IgG.
Specificity was confirmed by ELISA at less than 0.5 % of target signal.

Kreuzreaktivität: Maus

Produktmerkmale: Mouse IgG2b alkaline phosphatase conjugated secondary antibodies are ideal for western blot and are available in a variety of formats and conjugate types. When choosing a secondary antibody, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Aufreinigung: Preadsorption: Solid phase absorption

Sterilität: Sterile filtered

Immunogen:

Immunogen: Mouse IgG2b heavy chain

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:200 - 1:1,000

Application Note: Anti-Mouse IgG2b secondary antibody is suitable for ELISA, Immunohistochemistry, western blotting as well as other anti IgG2b antibody based assays. Mouse IgG2b alkaline phosphatase conjugated secondary antibodies are ideal for western blot and are available in a variety of formats and conjugate types.

ELISA Dilution: 1:2,000 - 1:10,000

Western Blot Dilution: 1:500 - 1:2,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed.
Centrifuge product if not completely clear after standing at room temperature.

Lagerung: 4 °C

Informationen zur Lagerung: Store vial at 4 °C prior to opening. This product is stable at 4 °C as an undiluted liquid. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -24 °C or below.

Haltbarkeit: 12 months

Antigendetails

Target: IgG2b

Abstract: IgG2b Produkte

Substanzklasse: Antibody

Hintergrund:

Synonyms: goat anti-MOUSE IgG2b Antibody Alkaline phosphatase Conjugation, goat anti-Mouse IgG2b (gamma 2b) alk phos Conjugated Antibody

Background: Anti-Mouse IgG2b Alkaline Phosphatase Antibody generated in goat detects reactivity to Mouse IgG2b (Gamma 2b chain). Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG2, the second largest of IgG isotypes, comprises almost 25 % of IgG and has a low affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.