Kaninchen anti-Human IgE Antikörper

Produktnummer ABIN1078163

Produktdetails

Target: IgE

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Applikation: ELISA, Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Spezifität: It has been selected for its ability to recognize IgE in immunohistochemical staining and Western blotting.

Aufreinigung: Affinity Chromatography

Immunogen: Native Protein IgE Immunoglobulin E (IgE) is a class of antibody that has been found only in mammals. IgE's main function is immunity to parasites such as parasitic worms. IgE also plays an essential role in the immediate hypersensitivity states, such as asthma, hay fever, atopic dermatitis, and other well-known manifestations of atopic patients. IgE is present in normal individuals at very low concentrations, only 0.05 % of the Ig concentration.
The antibody is a rabbit polyclonal antibody raised against IgE.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 200 µg/mL

Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handhabung: Avoid repeated freeze/thaw cycles

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.

Haltbarkeit: 12 months

Referenzen

Kim, Giri, Yun, Kim, Kim, Kang, Han, Kwon, Oh, Jun, Park: "Synergistic phage-surfactant combination clears IgE-promoted Staphylococcus aureus aggregation in vitro and enhances the effect in vivo." in: International journal of antimicrobial agents, Vol. 56, Issue 1, pp. 105997, (2020) (PubMed).

Antigendetails

Target: IgE

Abstract: IgE Produkte