Ziege anti-Maus IgG1 (Heavy Chain) Antikörper - Preadsorbed

Produktnummer ABIN1043977

Produktdetails

Target: IgG1

Bindungsspezifität: Heavy Chain

Reaktivität: Maus

Wirt: Ziege

Klonalität: Polyklonal

Applikation: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Spezifität: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum, Mouse IgG and Mouse IgG1. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA for human and rabbit. For IgG2a, IgG2b and IgG3 reactivity was less than 5% against target (IgG1, IgG). Cross-reactivity against Bovine is assessed only by IEP.

Kreuzreaktivität: Maus

Produktmerkmale: Mouse IgG1 secondary antibody is available in a variety of formats.

Aufreinigung: Preadsorption: Solid phase absorption

Sterilität: Sterile filtered

Immunogen:

Immunogen: Mouse IgG1 heavy chain

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:2,000 - 1:10,000

Application Note: Anti-Mouse IgG1 secondary antibody is suitable for ELISA, Immunohistochemistry western blotting as well as other anti IgG1 antibody based assays.

ELISA Dilution: 1:40,000 - 1:200,000

Western Blot Dilution: 1:4,000 - 1:20,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid cycles of freezing and thawing.
Centrifuge product if not completely clear after standing at room temperature.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store second antibody at 4 °C prior to restoration. For extended storage aliquot secondary antibody and freeze at -24 °C or below. This product is stable for several weeks at 4 °C as an undiluted liquid.

Haltbarkeit: 12 months

Antigendetails

Target: IgG1

Abstract: IgG1 Produkte

Substanzklasse: Antibody

Hintergrund:

Synonyms: Goat anti-mouse IgG1 antibody, goat anti-mouse IgG1 (gamma 1) antibody

Background: Anti-Mouse IgG1 Antibody generated in goat detects reactivity to Mouse IgG1 (Gamma 1 chain). Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG1 chain constitutes 66 % of the IgG subclass and has a high affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.