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Kaninchen anti-Cat IgM (Heavy Chain) Antikörper

ELISA, IHC, WB Polyclonal IgG unconjugated
Produktnummer ABIN102560
  • Target Alle IgM Produkte
    IgM
    Bindungsspezifität
    • 141
    • 117
    • 87
    • 41
    • 17
    • 8
    • 7
    • 1
    • 1
    Heavy Chain
    Reaktivität
    • 194
    • 134
    • 66
    • 39
    • 32
    • 26
    • 22
    • 21
    • 16
    • 15
    • 15
    • 14
    • 13
    • 13
    • 11
    • 4
    • 2
    • 1
    Cat
    Wirt
    • 321
    • 187
    • 80
    • 19
    • 14
    • 5
    • 4
    • 1
    • 1
    Kaninchen
    Klonalität
    • 495
    • 89
    Polyklonal
    Applikation
    • 337
    • 249
    • 241
    • 211
    • 144
    • 139
    • 64
    • 63
    • 47
    • 47
    • 47
    • 43
    • 38
    • 29
    • 20
    • 18
    • 13
    • 6
    • 5
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    Spezifität
    IgM µ chain
    Produktmerkmale
    Concentration Definition: by UV absorbance at 280 nm
    Aufreinigung
    This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Cat IgM and Cat Serum.  No reaction was observed against Cat IgG.
    Immunogen

    Immunogen: Cat IgM mu heavy chain

    Isotyp
    IgG
  • Applikationshinweise

    Immunohistochemistry Dilution: 1:1,000 - 1:5,000

    Application Note: Anti-Cat IgM antibody is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.

    ELISA Dilution: 1:20,000 - 1:100,000

    Western Blot Dilution: 1:2,000 - 1:10,000

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution

    Reconstitution Volume: 5.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Konzentration
    10.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide

    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    RT,4 °C,-20 °C
    Haltbarkeit
    12 months
  • Target
    IgM
    Abstract
    IgM Produkte
    Substanzklasse
    Antibody
    Hintergrund

    Synonyms: rabbit anti-Cat IgM mu Antibody, rabbit anti Cat IgM mu

    Background: Anti-Cat IgM antibody specifically detects cat IgM. Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900 kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum. Anti-Cat IgM antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.

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