Kaninchen anti-Human IgG (Fc Region) Antikörper (Alkaline Phosphatase (AP)) - Preadsorbed

Produktnummer ABIN101667

Produktdetails

Target: IgG

Bindungsspezifität: Fc Region

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Alkaline Phosphatase (AP)

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Spezifität: IgG F(c)

Kreuzreaktivität: Human

Produktmerkmale: Concentration Definition: by UV absorbance at 280 nm

Aufreinigung: Preadsorption: Solid phase absorption

Sterilität: Sterile filtered

Immunogen:

Immunogen: Anti-Human IgG F(c) was produced by repeated immunization with Human Fc fragment in rabbit.

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:200 - 1:1,000

Application Note: Anti-Human IgG F(c) antibody is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.

ELISA Dilution: 1:2,000 - 1:10,000

Western Blot Dilution: 1:500 - 1:2,500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed.

Lagerung: 4 °C

Haltbarkeit: 12 months

Antigendetails

Target: IgG

Abstract: IgG Produkte

Substanzklasse: Antibody

Hintergrund:

Synonyms: Human Fc Antibody, Rb-a-Human F(c), Human IgG F(c) Antibody in Rabbit, rabbit anti-Human IgG F(c) Alkaline phosphatase Secondary Antibody

Background: Anti-Human IgG F(c) Alkaline Phosphatase Conjugated generated in rabbit detects Human F(c). A proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH . Receptors bind the Fc portion of human IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(c) Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.