Ziege anti-Human IgG (Fc Region) Antikörper (Alkaline Phosphatase (AP)) - Preadsorbed
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- Target Alle IgG Produkte
- IgG
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Bindungsspezifität
- Fc Region
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Reaktivität
- Human
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Wirt
- Ziege
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Klonalität
- Polyklonal
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Konjugat
- Alkaline Phosphatase (AP)
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Applikation
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- Spezifität
- IgG F(c)
- Produktmerkmale
- Concentration Definition: by UV absorbance at 280 nm
- Aufreinigung
- Preadsorption: Solid phase absorption
- Sterilität
- Sterile filtered
- Immunogen
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Immunogen: Human IgG F(c) fragment
- Isotyp
- IgG
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- Applikationshinweise
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Immunohistochemistry Dilution: 1:200 - 1:1,000
Application Note: Anti-Human IgG F(c) Alk Phos conjugate is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency.
ELISA Dilution: 1:10,000 - 1:40,000
Western Blot Dilution: 1:1,000 - 1:5,000
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 1.0 mg/mL
- Buffer
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Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.1 % (w/v) Sodium Azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
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Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed. - Lagerung
- 4 °C
- Haltbarkeit
- 12 months
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- Target
- IgG
- Abstract
- IgG Produkte
- Substanzklasse
- Antibody
- Hintergrund
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Synonyms: goat anti-Human IgG F(c) alkaline phosphatase Conjugated Antibody, goat anti-Human IgG F(c) fragment antibody alk phos conjugation
Background: Anti-Human IgG F(c) Alkaline Phosphatase Conjugated generated in goat detects Human F(c). A proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH . Receptors bind the Fc portion of human IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(c) Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
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