Ziege anti-Maus IgA, IgG, IgM (Heavy & Light Chain) Antikörper (Biotin) - Preadsorbed
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- Target Alle IgA, IgG, IgM Produkte
- IgA, IgG, IgM
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Bindungsspezifität
- Heavy & Light Chain
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Reaktivität
- Maus
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Wirt
- Ziege
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Klonalität
- Polyklonal
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Konjugat
- Biotin
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Applikation
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- Spezifität
- IgG IgA IgM
- Kreuzreaktivität
- Maus
- Produktmerkmale
- Concentration Definition: by UV absorbance at 280 nm
- Aufreinigung
- Preadsorption: immunoaffinity chromatography using antigens coupled to agarose beads followed by cross adsorption to remove unwanted specificities
- Immunogen
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Immunogen: Mouse IgG IgA and IgM whole molecule
- Isotyp
- IgG
- Labeling Ratio
- 10-20
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- Applikationshinweise
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Immunohistochemistry Dilution: 1:1,500 - 1:15,000
Application Note: Immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
ELISA Dilution: 1:300,000
Western Blot Dilution: 1:3,000 - 1:30,000
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
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Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Konzentration
- 1.0 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Aliquot to Avoid repeated freezing and thawing.
- Lagerung
- RT,4 °C,-20 °C
- Haltbarkeit
- 12 months
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- Target
- IgA, IgG, IgM
- Andere Bezeichnung
- IgG IgA IgM (IgA, IgG, IgM Produkte)
- Substanzklasse
- Antibody
- Hintergrund
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Synonyms: Goat Anti Mouse IgG IgA IgM Antibody Biotin Conjugation, Goat Anti-Mouse IgG IgA IgM Biotin conjugated Antibody, Goat Anti-Mouse IgGAM Biotin conjugate
Background: Anti-Mouse IgG IgA IgM Biotin Antibody generated in goat detects reactivity to Mouse IgG, Mouse IgA, and Mouse IgM. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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