Ratte (Rattus) CLOCK ELISA Kit

Recommended CLOCK Product (geliefert von: Anmelden zum Anzeigen )

Antigen
Clock Homolog (Mouse) (CLOCK) ELISA Kits
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Reaktivität
Human, Maus, Ratte (Rattus)
Alternativen
Kits mit alternativen Reaktivitäten:
7
3
1
Methodentyp
Cell ELISA
Applikation
ELISA
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Produktnummer ABIN2113519
$ 493.51
Zzgl. Versandkosten $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Hersteller References Details
1 ABIN5648169 Cell ELISA Cell Culture Cells Anmelden zum Anzeigen

General

Antigen Clock Homolog (Mouse) (CLOCK) ELISA Kits
Reaktivität Human, Maus, Ratte (Rattus)
Kits mit alternativen Reaktivitäten:
(7), (3), (1)
Methodentyp Cell ELISA
Applikation ELISA
Hersteller Anmelden zum Anzeigen

Produktdetails CLOCK ELISA Kit

Antigendetails Anwendungsinformationen Handhabung Bilder
Proben Cell Culture Cells
Nachweismethode Colorimetric
Analytische Methode Quantitative
Produktmerkmale Assay Type: Cell-Based

Antigendetails

Produktdetails CLOCK ELISA Kit Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung Clock (CLOCK ELISA Kit Abstract)
Hintergrund Synonyms: circadian locomotor output cycles kaput, hCLOCK, KIAA0334
Gene Symbol: CLOCK
Gen-ID 9575
UniProt O15516
Pathways Regulation of Lipid Metabolism by PPARalpha, Photoperiodism

Anwendungsinformationen

Produktdetails CLOCK ELISA Kit Antigendetails Handhabung Bilder zurück nach oben
Plattentyp Uncoated
Testdurchführung
  • Remove the coating solution and wash the plate three times by filling the wells with 100 μL PBS-0.05 % Tween20. The solutions or washes are removed by flicking the plate over a sink. The remaining drops are removed by patting the plate on a paper towel.
  • Block the remaining protein-binding sites in the coated wells by adding 100 μL blocking buffer, 3 % skim milk in PBS per well. Incubate for 1 hour at RT with gentle shaking.
  • Wash the plate three times with 100 μL PBS-0.05 % Tween 20.
  • Add 50 μL of diluted antibody to each well. Incubate the plate at 37 °C for an hour with gentle shaking.
  • Wash the plate six times with 100 μL PBS-0.05 %Tween 20.
  • Add 50 μL of conjugated secondary antibody, diluted at the optimal concentration (according to the manufacturer) in blocking buffer immediately before use. Incubate at 37 °C for an hour.
  • Wash the plate six times with 100 μL PBS-0.05 %Tween20.
  • Prepare the substrate solution by mixing acetic acid, TMB and 0.03 % H2O2 with the volume ratio of 4:1:5.
  • Dispense 50 μL of the substrate solution per well with a multichannel pipe. Incubate the plate at 37 °C in dark for 15-30 mins.
  • After sufficient color development, add 100 μL of stop solution to the wells (if necessary).
  • Read the absorbance (optical density at 450nm) of each well with a plate reader.
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails CLOCK ELISA Kit Antigendetails Anwendungsinformationen Bilder zurück nach oben
Handhabung Avoid multiple freeze-thaw cycles
Lagerung 4 °C
Informationen zur Lagerung Store at 4 °C for frequent use, at -20°C for infrequent use.
Haltbarkeit 6 months